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Scientific article
Open access
English

Decontamination of 16S rRNA gene amplicon sequence datasets based on bacterial load assessment by qPCR

Published inBMC Microbiology, vol. 16, 73
Publication date2016
Abstract

Identification of unexpected taxa in 16S rRNA surveys of low-density microbiota, diluted mock communities and cultures demonstrated that a variable fraction of sequence reads originated from exogenous DNA. The sources of these contaminants are reagents used in DNA extraction, PCR, and next-generation sequencing library preparation, and human (skin, oral and respiratory) microbiota from the investigators.

Keywords
  • Bacteria/classification/genetics
  • Bacterial Load
  • Cluster Analysis
  • Computational Biology/methods
  • Computer Simulation
  • DNA Contamination
  • DNA, Bacterial/genetics
  • DNA, Ribosomal/genetics
  • Humans
  • Microbiota
  • Polymerase Chain Reaction/methods
  • RNA, Ribosomal, 16S/genetics
  • Sequence Analysis, DNA
Citation (ISO format)
LAZAREVIC, Vladimir et al. Decontamination of 16S rRNA gene amplicon sequence datasets based on bacterial load assessment by qPCR. In: BMC Microbiology, 2016, vol. 16, p. 73. doi: 10.1186/s12866-016-0689-4
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal1471-2180
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168downloads

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