• Home
  1. Home
  2. Comprehensive metagenomic analysis of glioblastoma reveals absence of known virus despite antiviral-like type I interferon gene response
Scientific article
OA Policy
English

Comprehensive metagenomic analysis of glioblastoma reveals absence of known virus despite antiviral-like type I interferon gene response

Contributors

Cosset, Erika; Petty Ii, Thomas John; Dutoit Vallotton, Valérie; Cordey, Samuel; Padioleau, Ismael; Otten, Patricia; Farinelli, Laurent Luca Denis; Kaiser, Laurent; Bruyère-Cerdan, Pascale; Tirefort, Diderik; Amar El-Dusouqui, Soraya; Nayernia, Zeynab; Krause, Karl-Heinz; Zdobnov, Evgeny; Dietrich, Pierre-Yves; Rigal, Emmanuel; Preynat-Seauve, Olivier

Published in

International journal of cancer, vol. 135, no. 6, p. 1381-1389

Publication date

2014

Abstract

Glioblastoma is a deadly malignant brain tumor and one of the most incurable forms of cancer in need of new therapeutic targets. As some cancers are known to be caused by a virus, the discovery of viruses could open the possibility to treat, and perhaps prevent, such a disease. Although an association with viruses such as cytomegalovirus or Simian virus 40 has been strongly suggested, involvement of these and other viruses in the initiation and/or propagation of glioblastoma remains vague, controversial and warrants elucidation. To exhaustively address the association of virus and glioblastoma, we developed and validated a robust metagenomic approach to analyze patient biopsies via high-throughput sequencing, a sensitive tool for virus screening. In addition to traditional clinical diagnostics, glioblastoma biopsies were deep-sequenced and analyzed with a multistage computational pipeline to identify known or potentially discover unknown viruses. In contrast to the studies reporting the presence of viral signatures in glioblastoma, no common or recurring active viruses were detected, despite finding an antiviral-like type I interferon response in some specimens. Our findings highlight a discrete and non-specific viral signature and uncharacterized short RNA sequences in glioblastoma. This study provides new insights into glioblastoma pathogenesis and defines a general methodology that can be used for high-resolution virus screening and discovery in human cancers.

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

COSSET, Erika et al. Comprehensive metagenomic analysis of glioblastoma reveals absence of known virus despite antiviral-like type I interferon gene response. In: International journal of cancer, 2014, vol. 135, n° 6, p. 1381–1389. doi: 10.1002/ijc.28670

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0020-7136
771views
538downloads

Technical informations

Creation

04/09/2014 13:11:00

First validation

04/09/2014 13:11:00

Update

09/02/2026 15:39:40

Status update

09/02/2026 15:39:40

Last indexation

26/03/2026 14:45:25
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated