Comprehensive metagenomic analysis of glioblastoma reveals absence of known virus despite antiviral-like type I interferon gene response

Article Article scientifique Article Comprehensive metagenomic analysis of glioblastoma reveals absence of known virus despite antiviral-like type I interferon gene response eng 2014 author Cosset Erika 758924 author Petty Ii Thomas John 645271 author Dutoit Vallotton Valérie 0000-0002-3469-4237 668630 author Cordey Samuel 507357 author Padioleau Ismael 739561 author Otten Patricia 680482 author Farinelli Laurent Luca Denis 23905 author Kaiser Laurent 28229 author Bruyère-Cerdan Pascale author Tirefort Diderik 134979 author Amar El-Dusouqui Soraya author Nayernia Zeynab 709368 author Krause Karl-Heinz 23022 author Zdobnov Evgeny 636914 author Dietrich Pierre-Yves 96532 author Rigal Emmanuel 743436 author Preynat-Seauve Olivier 561776 1381-1389 10.1002/ijc.28670 24347514 0020-7136 Glioblastoma is a deadly malignant brain tumor and one of the most incurable forms of cancer in need of new therapeutic targets. As some cancers are known to be caused by a virus, the discovery of viruses could open the possibility to treat, and perhaps prevent, such a disease. Although an association with viruses such as cytomegalovirus or Simian virus 40 has been strongly suggested, involvement of these and other viruses in the initiation and/or propagation of glioblastoma remains vague, controversial and warrants elucidation. To exhaustively address the association of virus and glioblastoma, we developed and validated a robust metagenomic approach to analyze patient biopsies via high-throughput sequencing, a sensitive tool for virus screening. In addition to traditional clinical diagnostics, glioblastoma biopsies were deep-sequenced and analyzed with a multistage computational pipeline to identify known or potentially discover unknown viruses. In contrast to the studies reporting the presence of viral signatures in glioblastoma, no common or recurring active viruses were detected, despite finding an antiviral-like type I interferon response in some specimens. Our findings highlight a discrete and non-specific viral signature and uncharacterized short RNA sequences in glioblastoma. This study provides new insights into glioblastoma pathogenesis and defines a general methodology that can be used for high-resolution virus screening and discovery in human cancers. Dewey 616 Dewey 616.07 Dewey 576.5 135 Département de médecine génétique et développement 21D 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 false 400 Virologie clinique 668 493 Génomique Evolutionnaire Computationnelle 830 27 Immunothérapie des cancers 42 38 Radicaux libres et cellules souches embryonnaires 60 International journal of cancer 135 6 6fe92417-766d-432c-924f-59f7cc5e1a0f Article (Published version) Public ECosset_Comprehensive metagenomic analysis glioblastoma.pdf 652354 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine

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Comprehensive metagenomic analysis of glioblastoma reveals absence of known virus despite antiviral-like type I interferon gene response	International journal of cancer			2014	757	496

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