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Title

Challenges in the culture-independent analysis of oral and respiratory samples from intubated patients

Authors
Yugueros Marcos, Javier
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Published in Frontiers in Cellular and Infection Microbiology. 2014, vol. 4, p. 65
Abstract The spread of microorganisms in hospitals is an important public health threat, and yet few studies have assessed how human microbial communities (microbiota) evolve in the hospital setting. Studies conducted so far have mainly focused on a limited number of bacterial species, mostly pathogenic ones and primarily during outbreaks. We explored the bacterial community diversity of the microbiota from oral and respiratory samples of intubated patients hospitalized in the intensive care unit and we discuss the technical challenges that may arise while using culture-independent approaches to study these types of samples.
Keywords BiotaHumansIntensive Care UnitsIntubation, IntratrachealMicrobiological Techniques/methodsMicrobiotaMolecular Biology/methodsMouth/microbiologyPilot ProjectsRNA, Ribosomal, 16S/geneticsRespiratory System/microbiology
Identifiers
PMID: 24904840
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Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Groupe Pugin Jérôme (Soins intensifs) (587)
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LAZAREVIC, Vladimir et al. Challenges in the culture-independent analysis of oral and respiratory samples from intubated patients. In: Frontiers in Cellular and Infection Microbiology, 2014, vol. 4, p. 65. https://archive-ouverte.unige.ch/unige:42448

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Deposited on : 2014-12-03

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