UNIGE document Scientific Article
previous document  unige:88247  next document
add to browser collection
Title

Aminoglycoside analysis in food of animal origin with a zwitterionic stationary phase and liquid chromatography–tandem mass spectrometry

Authors
Published in Analytica Chimica Acta. 2015, vol. 882, p. 127-139
Abstract In this study, fourteen highly polar aminoglycoside (AGs) antibiotics were selected. Various stationary phases were tested, including Obelisc R, ZIC-HILIC, BEH amide and aminopropyl. The nature of the stationary phase, mobile phase (water or buffer solutions and acetonitrile), pH (percentage of formic acid), gradient conditions and injection solvents were systematically studied as relevant parameters for tuning retention selectivity and detectability of AGs in liquid chromatography electrospray tandem mass spectrometry (LC–(ESI)–MS/MS). Only the two zwitterionic phases (Obelisc R and ZIC-HILIC) achieved a proper chromatographic separation considering interferences due to the crosstalk effect in low resolution mass spectrometers. The water/acetonitrile mobile phase containing 1% formic acid used with Obelisc R provided more sensitivity than the highly concentrated buffered mobile phases required for ZIC-HILIC. A solid phase extraction (SPE) clean-up procedure with polymeric weak cation exchange (WCX) cartridges was optimized for honey, milk and liver samples. Different brands of cartridges and elution solvents were tested, and the Taurus WCX offered the best recovery rate with a buffer elution at pH 3. The final optimized method was validated in these matrices according to Decision 2002/657/EC. A monitoring campaign for sixty honey, milk and liver samples was carried out at the Food Authority
Keywords AminoglycosidesZwitterionicHoneyMilkLiver
Full text
Article (Published version) (1.5 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://dx.doi.org/10.1016/j.aca.2015.03.050
Structures
Research group Sciences analytiques
Citation
(ISO format)
DIEZ, Cristina et al. Aminoglycoside analysis in food of animal origin with a zwitterionic stationary phase and liquid chromatography–tandem mass spectrometry. In: Analytica Chimica Acta, 2015, vol. 882, p. 127-139. https://archive-ouverte.unige.ch/unige:88247

133 hits

0 download

Update

Deposited on : 2016-10-17

Export document
Format :
Citation style :