Aminoglycoside analysis in food of animal origin with a zwitterionic stationary phase and liquid chromatography

Article Article scientifique Article Aminoglycoside analysis in food of animal origin with a zwitterionic stationary phase and liquid chromatography–tandem mass spectrometry eng 2015 author Diez Cristina 787018 author Guillarme Davy 623637 author Staub Sporri Aline 526793 author Cognard Emmanuelle 90119 author Ortelli Didier 86161 author Edder Patrick 26675 author Rudaz Serge 0000-0002-4180-5417 28978 127-139 10.1016/j.aca.2015.03.050 26043099 0003-2670 In this study, fourteen highly polar aminoglycoside (AGs) antibiotics were selected. Various stationary phases were tested, including Obelisc R, ZIC-HILIC, BEH amide and aminopropyl. The nature of the stationary phase, mobile phase (water or buffer solutions and acetonitrile), pH (percentage of formic acid), gradient conditions and injection solvents were systematically studied as relevant parameters for tuning retention selectivity and detectability of AGs in liquid chromatography electrospray tandem mass spectrometry (LC–(ESI)–MS/MS). Only the two zwitterionic phases (Obelisc R and ZIC-HILIC) achieved a proper chromatographic separation considering interferences due to the crosstalk effect in low resolution mass spectrometers. The water/acetonitrile mobile phase containing 1% formic acid used with Obelisc R provided more sensitivity than the highly concentrated buffered mobile phases required for ZIC-HILIC. A solid phase extraction (SPE) clean-up procedure with polymeric weak cation exchange (WCX) cartridges was optimized for honey, milk and liver samples. Different brands of cartridges and elution solvents were tested, and the Taurus WCX offered the best recovery rate with a buffer elution at pH 3. The final optimized method was validated in these matrices according to Decision 2002/657/EC. A monitoring campaign for sixty honey, milk and liver samples was carried out at the Food Authority Aminoglycosides Zwitterionic Honey Milk Liver Dewey 615 31 Section des sciences pharmaceutiques 17 false 881 Sciences analytiques Analytica chimica acta 882 580bea01-0060-4e84-bc73-324b3755aaa5 Article (Published version) Restricted 2015(4).pdf 1526090 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Aminoglycoside analysis in food of animal origin with a zwitterionic stationary phase and liquid chromatography–tandem mass spectrometry	Analytica chimica acta			2015	565	1

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Cognard, Emmanuelle