Scientific article

Electron currents generated by the human phagocyte NADPH oxidase

Published inNature, vol. 392, no. 6677, p. 734-737
Publication date1998

Electron transport across biological membranes is a well-known feature of bacteria, mitochondria and chloroplasts, where it provides motive forces for vectorial transport processes. In contrast, electron transport is generally not found in the plasma membrane of eukaryotic cells, possibly because it would interfere with electric processes at the plasma membrane. An exception is provided by the phagocyte NADPH oxidase, which generates superoxide (O2.-) through electron transfer from cytosolic NADPH to extracellular oxygen. The enzyme is essential for host defence, and patients with chronic granulomatous disease, who lack the functional enzyme, suffer from severe infections. It has been suggested that electron transfer by the NADPH oxidase might be electrogenic. Here we demonstrate, using the whole-cell patch-clamp technique, the generation of electron currents by the NADPH oxidase in human eosinophil granulocytes. The currents were absent in granulocytes of sufferers of chronic granulomatous disease and under conditions of low oxygen. Generation of electron currents across the plasma membrane of eukaryotic cells has not been observed previously and might be-independently of the generation of superoxide-a physiologically relevant function of the phagocyte NADPH oxidase.

  • Adult
  • Electron Transport
  • Eosinophils/ enzymology
  • Female
  • Glucosephosphate Dehydrogenase Deficiency/metabolism
  • Granulomatous Disease, Chronic/metabolism
  • Heterozygote
  • Humans
  • Male
  • NADPH Oxidase/ metabolism
  • Oxygen/metabolism
  • Patch-Clamp Techniques
  • Phagocytes/ enzymology
Citation (ISO format)
SCHRENZEL, Jacques et al. Electron currents generated by the human phagocyte NADPH oxidase. In: Nature, 1998, vol. 392, n° 6677, p. 734–737. doi: 10.1038/33725
Updates (1)
ISSN of the journal0028-0836

Technical informations

Creation06/21/2010 10:26:24 AM
First validation06/21/2010 10:26:24 AM
Update time03/14/2023 3:45:34 PM
Status update03/14/2023 3:45:33 PM
Last indexation10/18/2023 9:29:14 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack