UNIGE document Scientific Article - Meta-analysis
previous document  unige:21421  next document
add to browser collection
Title

Intrapartum Group B streptococcus detection by rapid polymerase chain reaction assay for the prevention of neonatal sepsis

Authors
Published in Clinical Microbiology and Infection. 2011, vol. 17, no. 12, p. 1786-91
Abstract Group B streptococcus (GBS) is a leading cause of infectious neonatal morbidity and mortality. Timely and accurate identification of colonized mothers is imperative so that antibioprophylaxis can be implemented during labour to reduce the risk of neonatal sepsis. We planned our study to analyse the diagnostic accuracy of an intrapartum PCR assay to identify GBS-colonized women and to allow the implementation of correct (i.e. at least 4 h) intrapartum antibiotic prophylaxis based on the PCR results. We included 695 women in labour who were tested for rectovaginal GBS carriage by culture and PCR. Women were also screened at 35-37 weeks of gestation. Intrapartum GBS colonization was 19.3%. Assay sensitivity was 81.0% for antenatal culture and 85.0% for intrapartum PCR; p 0.72. GBS colonization (n = 107) was known at least 4 h before delivery in 68 (64%) and 73 (68%) women based on antenatal culture and intrapartum PCR, respectively. Among 43 women delivering preterm, correct status was known at least 4 h before delivery in 10 (23%) and 32 (74%) women according to antenatal culture and intrapartum PCR, respectively. These results support the concept that GBS screening can be performed routinely during labour in a clinical setting. The intrapartum approach is at least as accurate as the antenatal screening, with the additional advantage of identifying women delivering preterm or not followed during pregnancy.
Keywords AdultBacteriological Techniques/methodsCarrier State/diagnosis/microbiologyFemaleHumansMass Screening/methodsMolecular Diagnostic Techniques/methodsPerineum/microbiologyPolymerase Chain Reaction/methodsPregnancyPregnancy Complications, Infectious/diagnosis/microbiologyProspective StudiesRectum/microbiologySensitivity and SpecificitySepsis/prevention & controlStreptococcal Infections/diagnosis/microbiology/prevention & controlStreptococcus agalactiae/isolation & purificationVagina/microbiology
Identifiers
PMID: 20860701
Full text
Article (Published version) (98 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Réanimation néonatale en salle d'accouchement (696)
Citation
(ISO format)
MARTINEZ DE TEJADA WEBER, Begona et al. Intrapartum Group B streptococcus detection by rapid polymerase chain reaction assay for the prevention of neonatal sepsis. In: Clinical Microbiology and Infection, 2011, vol. 17, n° 12, p. 1786-91. https://archive-ouverte.unige.ch/unige:21421

198 hits

0 download

Update

Deposited on : 2012-05-25

Export document
Format :
Citation style :