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Title

Methicillin-resistant coagulase-negative staphylococci in the community: high homology of SCCmec IVa between Staphylococcus epidermidis and major clones of methicillin-resistant Staphylococcus aureus

Authors
Barbier, Francois
Ruppe, Etienne
Lebeaux, David
Felix, Benjamin
Desprez, Adeline
Maiga, Aminata
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Published in Journal of Infectious Diseases. 2010, vol. 202, no. 2, p. 270-281
Abstract BACKGROUND: Data on community spread of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are scarce. We assessed their potential role as a reservoir of staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). METHODS: Nasal carriage of MR-CoNS was prospectively investigated in 291 adults at hospital admission. MR-CoNS were characterized by SCCmec typing, long-range polymerase chain reaction (PCR) for SCCmec IV, and multiple-locus variable-number tandem repeat analysis (MLVA) for Staphylococcus epidermidis (MRSE) strains. Three SCCmec IVa elements were fully sequenced. RESULTS: The carriage rate of MR-CoNS was 19.2% (25.9% and 16.5% in patients with and patients without previous exposure to the health care system, respectively; P = .09). MR-CoNS strains (n = 83, including 58 MRSE strains with highly heterogeneous MLVA patterns) carried SCCmec type IVa (n = 9, all MRSE), other SCCmec IV subtypes (n = 9, including 7 MRSE), other SCCmec types (n = 15), and nontypeable SCCmec (n = 50). Long-range PCR indicated structural homology between SCCmec IV in MRSE and that in MRSA. Complete sequences of SCCmec IVa from 3 MRSE strains were highly homologous to those available for CA-MRSA, including major clones USA300 and USA400. CONCLUSIONS: MR-CoNS are probably disseminated in the community, notably in subjects without previous exposure to the health care system. MRSE, the most prevalent species, may act as a reservoir of SCCmec IVa for CA-MRSA.
Keywords AdolescentAdultAgedAged, 80 and overBase SequenceClone CellsCoagulase/deficiency/*geneticsDNA PrimersDisease Reservoirs/microbiologyDrug Resistance, Bacterial/immunologyFemaleHospitalization/statistics & numerical dataHumansImmunoglobulin AllotypesMaleMethicillin Resistance/genetics/*physiologyMiddle AgedNose/microbiologyPolymerase Chain ReactionRenal DialysisStaphylococcal Infections/*epidemiology/immunology/transmissionStaphylococcus epidermidis/*enzymology/*genetics
Identifiers
PMID: 20550456
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Other version: http://jid.oxfordjournals.org/content/202/2/270.full.pdf
Structures
Research group Analyse génomique et fonctionnelle du staphylocoque doré (604)
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BARBIER, Francois et al. Methicillin-resistant coagulase-negative staphylococci in the community: high homology of SCCmec IVa between Staphylococcus epidermidis and major clones of methicillin-resistant Staphylococcus aureus. In: Journal of Infectious Diseases, 2010, vol. 202, n° 2, p. 270-281. https://archive-ouverte.unige.ch/unige:20758

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Deposited on : 2012-05-23

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