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Next-Generation Sequencing Techniques to Diagnose Culture-Negative Subacute Native Aortic Endocarditis

Published inEmerging infectious diseases, vol. 31, no. 7, p. 1478-1481
Publication date2025-07
Abstract

Next-generation sequencing might improve diagnosis of infective endocarditis. A case in Switzerland was initially attributed to Solobacterium moorei bacteria. Metagenomic analysis of the affected heart valve detected Streptococcus gordonii, but not S. moorei, illustrating that the results of molecular detection can vary depending on sampling time and anatomic site.

Keywords
  • DNA
  • Switzerland
  • Bacteremia
  • Bacteria
  • Cell-free nucleic acid
  • Endocarditis
  • Infective endocarditis
  • McfDNA
  • Sequence analysis
  • Streptococci
  • Humans
  • Male
  • Aortic Valve / microbiology
  • Endocarditis / diagnosis
  • Endocarditis / microbiology
  • Endocarditis, Bacterial / diagnosis
  • Endocarditis, Bacterial / microbiology
  • High-Throughput Nucleotide Sequencing / methods
  • Streptococcus gordonii / genetics
  • Streptococcus gordonii / isolation & purification
  • Aged, 80 and over
Citation (ISO format)
VETTERLI, Delphine et al. Next-Generation Sequencing Techniques to Diagnose Culture-Negative Subacute Native Aortic Endocarditis. In: Emerging infectious diseases, 2025, vol. 31, n° 7, p. 1478–1481. doi: 10.3201/eid3107.241739
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Article (Published version)
accessLevelPublic
Identifiers
Journal ISSN1080-6040
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196downloads

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Creation29/07/2025 14:41:38
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Update05/08/2025 07:47:18
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