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Title

Comparative transcriptomic and functional assessments of linezolid-responsive small RNA genes in staphylococcus aureus

Authors
Guérillot, Romain
Lin, Ya Hsun
Tree, Jai
Monk, Ian R.
Seemann, Torsten
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Published in mSystems. 2020, vol. 5, no. 1, p. e00665-19
Abstract Staphylococcus aureus contains a repertoire of at least 50 and possibly 500 small RNAs (sRNAs). The functions of most sRNAs are not understood, although some are known to respond to environmental changes, including the presence of antibiotics. Here, in an effort to better understand the roles of sRNAs in the context of antibiotic exposure, we took a clinical methicillin-resistant S. aureus (MRSA) isolate and separately deleted eight sRNAs that were significantly upregulated in response to the last-line antibiotic linezolid as revealed by transcriptome sequencing (RNA-seq) comparisons. We also deleted an additional 10 sRNAs that were either highly expressed or previously found to respond to antibiotic exposure. There were no significant changes for any of the 18 mutants in a variety of phenotypic screens, including MIC screens, growth competition assays in the presence of linezolid, biofilm formation, and resistance to whole-blood killing. These data suggest sRNA functional redundancy, because despite their high expression levels upon antibiotic exposure, individual sRNA genes do not affect readily observable bacterial phenotypes. The sRNA transcriptional changes we measured during antibiotic exposure might also reflect sRNA "indifference," that is, a general stress response not specifically related to sRNA function.
Keywords RNA sequencingStaphylococcus aureusMutagenesisPostantibiotic effectsSRNATranscriptome
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PMID: 31911464
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Research group Analyse génomique et fonctionnelle du staphylocoque doré (604)
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GAO, Wei et al. Comparative transcriptomic and functional assessments of linezolid-responsive small RNA genes in staphylococcus aureus. In: mSystems, 2020, vol. 5, n° 1, p. e00665-19. doi: 10.1128/mSystems.00665-19 https://archive-ouverte.unige.ch/unige:140133

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Deposited on : 2020-08-24

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