Comparative transcriptomic and functional assessments of linezolid-responsive small RNA genes in staphylococcus aureus

Article Article scientifique Article Comparative transcriptomic and functional assessments of linezolid-responsive small RNA genes in staphylococcus aureus eng 2020 author Gao Wei 596641 author Guérillot Romain author Lin Ya Hsun author Tree Jai author Beaume Marie-Emilie 684753 author Francois Patrice 0000-0002-0540-750X 28911 author Monk Ian R. author Seemann Torsten author Schrenzel Jacques 29184 author Howden Benjamin Peter 796943 author Stinear Timothy P. e00665-19 10.1128/mSystems.00665-19 31911464 2379-5077 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946794/ Staphylococcus aureus contains a repertoire of at least 50 and possibly 500 small RNAs (sRNAs). The functions of most sRNAs are not understood, although some are known to respond to environmental changes, including the presence of antibiotics. Here, in an effort to better understand the roles of sRNAs in the context of antibiotic exposure, we took a clinical methicillin-resistant S. aureus (MRSA) isolate and separately deleted eight sRNAs that were significantly upregulated in response to the last-line antibiotic linezolid as revealed by transcriptome sequencing (RNA-seq) comparisons. We also deleted an additional 10 sRNAs that were either highly expressed or previously found to respond to antibiotic exposure. There were no significant changes for any of the 18 mutants in a variety of phenotypic screens, including MIC screens, growth competition assays in the presence of linezolid, biofilm formation, and resistance to whole-blood killing. These data suggest sRNA functional redundancy, because despite their high expression levels upon antibiotic exposure, individual sRNA genes do not affect readily observable bacterial phenotypes. The sRNA transcriptional changes we measured during antibiotic exposure might also reflect sRNA "indifference," that is, a general stress response not specifically related to sRNA function. RNA sequencing Staphylococcus aureus Mutagenesis Postantibiotic effects SRNA Transcriptome Dewey 616 56 Département de médecine 221 false 338 Génomique et microbiote 604 mSystems 5 1 bd345abe-fa63-407e-b274-98ad4430e885 Article (Published version) Public COMPARATIVE.pdf 1441817 application/pdf CC BY openAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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Comparative transcriptomic and functional assessments of linezolid-responsive small RNA genes in staphylococcus aureus	mSystems			2020	363	249

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Gao, Wei