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Title

Palmitate and oleate modify membrane fluidity and kinase activities of INS-1E β-cells alongside altered metabolism-secretion coupling

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Published in Biochimica et Biophysica Acta - Molecular Cell Research. 2020, vol. 1867, no. 2, p. 118619
Abstract Chronic exposure to elevated levels of glucose and free fatty acids impairs beta-cell function, leading to insulin secretion defects and eventually beta-cell failure. Using a semi-high throughput approach applied to INS-1E beta-cells, we tested multiple conditions of chronic exposure to basal, intermediate and high glucose, combined with saturated versus mono- and polyunsaturated fatty acids in order to assess cell integrity, lipid metabolism, mitochondrial function, glucose-stimulated calcium rise and secretory kinetics. INS-1E beta-cells were cultured for 3 days at different glucose concentrations (5.5, 11.1, 25 mM) without or with BSA-complexed 0.4 mM saturated (C16:0 palmitate), monounsaturated (C18:1 oleate) or polyunsaturated (C18:2 linoleate, C18:3 linolenate) fatty acids, resulting in 0.1–0.5 μM unbound fatty acids. Accumulation of triglycerides in cells exposed to fatty acids was glucose-dependent, oleate inducing the strongest lipid storage and protecting against glucose-induced cytotoxicity. The combined chronic exposure to both high glucose and either palmitate or oleate altered mitochondrial function as well as glucose-induced calcium rise. This pattern did not directly translate at the secretory level since palmitate and oleate exhibited distinct effects on the first and the second phases of glucose-stimulated exocytosis. Both fatty acids changed the activity of kinases, such as the MODY-associated BLK. Additionally, chronic exposure to fatty acids modified membrane physicochemical properties by increasing membrane fluidity, oleate exhibiting larger effects compared to palmitate. Chronic fatty acids differentially and specifically exacerbated some of the glucotoxic effects, without promoting cytotoxicity on their own. Each of the tested fatty acids functionally modified INS-1E beta-cell, oleate inducing the strongest effects.
Keywords Fatty acidsGlucoseInsulinMembrane/fluidityPancreasLipotoxicity
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PMID: 31816355
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Other version: https://linkinghub.elsevier.com/retrieve/pii/S0167488919302277
Structures
Research groups Groupe Matile
Groupe Roux
Mitochondries et sécrétion d'insuline (671)
La transplantation d'îlots de Langerhans (623)
Projects FNS: 131003A_149975; 131003A_173087
FNS: 51NF40-160589; 31003A_166625
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(ISO format)
OBERHAUSER, Lucie et al. Palmitate and oleate modify membrane fluidity and kinase activities of INS-1E β-cells alongside altered metabolism-secretion coupling. In: Biochimica et Biophysica Acta - Molecular Cell Research, 2020, vol. 1867, n° 2, p. 118619. doi: 10.1016/j.bbamcr.2019.118619 https://archive-ouverte.unige.ch/unige:127987

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Deposited on : 2019-12-17

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