Isolation of pancreatic islets and primary culture of the intact microorgans or of dispersed islet cells
ContributorsWollheim, Claes; Meda, Paolo; Halban, Philippe A.
Published inMethods in enzymology, vol. 192, p. 188-223
Publication date1990
Keywords
- Animals
- Animals, Newborn
- Cell Aggregation
- Cell Division
- Cell Separation/methods
- Cells, Cultured
- Centrifugation, Density Gradient
- Culture Media
- Culture Techniques/methods
- DNA Replication
- Indicators and Reagents
- Insulin/secretion
- Islets of Langerhans/ cytology/secretion/ultrastructure
- Microbial Collagenase
- Microscopy, Electron, Scanning
- Pancreas/cytology
- Rats
Affiliation entities
Citation (ISO format)
WOLLHEIM, Claes, MEDA, Paolo, HALBAN, Philippe A. Isolation of pancreatic islets and primary culture of the intact microorgans or of dispersed islet cells. In: Methods in enzymology, 1990, vol. 192, p. 188–223. doi: 10.1016/0076-6879(90)92071-k
Identifiers
- PID : unige:9281
- DOI : 10.1016/0076-6879(90)92071-k
- PMID : 1963663
Journal ISSN0076-6879
