Isolation of pancreatic islets and primary culture of the intact microorgans or of dispersed islet cells
ContributorsWollheim, Claes; Meda, Paolo; Halban, Philippe A.
Published inMethods in enzymology, vol. 192, p. 188-223
Publication date1990
Keywords
- Animals
- Animals, Newborn
- Cell Aggregation
- Cell Division
- Cell Separation/methods
- Cells, Cultured
- Centrifugation, Density Gradient
- Culture Media
- Culture Techniques/methods
- DNA Replication
- Indicators and Reagents
- Insulin/secretion
- Islets of Langerhans/ cytology/secretion/ultrastructure
- Microbial Collagenase
- Microscopy, Electron, Scanning
- Pancreas/cytology
- Rats
UNIGE affiliation entities
Citation (ISO format)
WOLLHEIM, Claes, MEDA, Paolo, HALBAN, Philippe A. Isolation of pancreatic islets and primary culture of the intact microorgans or of dispersed islet cells. In: Methods in enzymology, 1990, vol. 192, p. 188–223. doi: 10.1016/0076-6879(90)92071-k
Identifiers
- PID : unige:9281
- DOI : 10.1016/0076-6879(90)92071-k
- PMID : 1963663
Journal ISSN0076-6879
