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Differential expression of E-cadherin at the surface of rat beta-cells as a marker of functional heterogeneity

Published in Journal of Endocrinology. 2007, vol. 194, no. 1, p. 21-29
Abstract The aim of this study was to assess whether the expression of E-cadherin at the surface of rat beta-cells is regulated by insulin secretagogues and correlates with insulin secretion. When cultured under standard conditions, virtually all beta-cells expressed E-cadherin observed by immunofluorescence, but heterogeneous staining was observed. Using fluorescence-activated cell sorting (FACS), two beta-cell sub-populations were sorted: one that was poorly labeled ('ECad-low') and another that was highly labeled ('ECad-high'). After 1-h stimulation with 16.7 mM glucose, insulin secretion (reverse hemolytic plaque assay) from individual ECad-high beta-cells was higher than that from ECad-low beta-cells. Ca2+-dependent beta-cell aggregation was increased at 16.7 mM glucose when compared with 2.8 mM glucose. E-cadherin at the surface of beta-cells was increased after 18 h at 11.1 and 22.2 mM glucose when compared with 2.8 mM glucose, with the greatest increase at 22.2 mM glucose + 0.5 mM isobutylmethylxanthine (IBMX). While no labeling was detected on freshly trypsinized cells, the proportion of stained cells increased in a time-dependent manner during culture for 1, 3, and 24 h. This recovery was faster when cells were incubated at 16.7 vs 2.8 mM glucose. Cycloheximide inhibited expression of E-cadherin at 2.8 mM glucose, but not at 16.7 mM, while depolymerization of actin by either cytochalasin B or latrunculin B increased surface E-cadherin at low glucose. In conclusion, these results show that expression of E-cadherin at the surface of islet beta-cells is controlled by secretagogues including glucose, correlates with insulin secretion, and can serve as a surface marker of beta-cell function.
Keywords Actins/metabolismAnimalsBicyclo Compounds, Heterocyclic/pharmacologyBiological Markers/analysisCadherins/analysis/ metabolismCells, CulturedCycloheximide/pharmacologyCytochalasin B/pharmacologyCytotoxins/pharmacologyFlow CytometryFluorescent Antibody TechniqueGlucose/metabolism/pharmacologyInsulin/ secretionIslets of Langerhans/chemistry/ metabolismMaleMarine Toxins/pharmacologyPhosphodiesterase Inhibitors/pharmacologyProtein Synthesis Inhibitors/pharmacologyRatsRats, Sprague-DawleyThiazolidines/pharmacologyTime Factors
PMID: 17592017
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BOSCO, Domenico, ROUILLER, Dominique, HALBAN, Philippe A. Differential expression of E-cadherin at the surface of rat beta-cells as a marker of functional heterogeneity. In: Journal of Endocrinology, 2007, vol. 194, n° 1, p. 21-29. doi: 10.1677/JOE-06-0169 https://archive-ouverte.unige.ch/unige:8653

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Deposited on : 2010-07-12

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