UNIGE document Scientific Article
previous document  unige:853  next document
add to browser collection

Rapid and high-throughput genotyping of Staphylococcus epidermidis isolates by automated multilocus variable-number of tandem repeats: a tool for real-time epidemiology

Hochmann, Alena
Klingenberg, Claus
show hidden authors show all authors [1 - 9]
Published in Journal of microbiological methods. 2008, vol. 72, no. 3, p. 296-305
Abstract Fast and reliable genotyping methods allowing real-time epidemiology would be instrumental to discriminate Staphylococcus epidermidis isolates, in order to evaluate potential cross-infections or to follow genome content of infecting strains of this important opportunistic pathogen. We describe an automated multilocus variable-number tandem repeat-based assay (MLVA) for the rapid genotyping of S. epidermidis. Multiplex PCR amplifications using 6 primer pairs targeting gene-regions containing variable numbers of tandem repeats and the mecA gene are resolved by micro-capillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for discriminatory power and reproducibility on 2 sequenced strains, on a collection of 21 strains previously characterized using genotyping reference methods and finally on 65 clinical isolates identified in two different institutions. All steps of this new procedure were developed to ensure rapid turn-around time and moderate costs. Our results suggest that this rapid approach is a valuable epidemiological tool to genotype S. epidermidis isolates in real-time. The rapid analysis of a limited number of evolutionary markers showed a power of discrimination similar to that of pulse-field gel electrophoresis (PFGE) or multilocus sequence type (MLST). This type of rapid and high-throughput methodology opens the possibility to rapidly assess long-term nosocomial transmission or to characterize infecting strains in the general procedure of routine laboratories, in real-time.
Keywords Anti-Bacterial Agents/pharmacologyBacterial Proteins/geneticsBacterial Typing Techniques/methodsCluster AnalysisDNA Bacterial/geneticsElectrophoresis CapillaryEpidemiology Molecular/*methodsGenotypeHumansMicrobial Sensitivity TestsMinisatellite RepeatsStaphylococcal Infections/epidemiology/microbiologyStaphylococcus epidermidis/classification/genetics/isolation & purification
PMID: 18237794
Full text
Article (Accepted version) (739 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Epidémiologie et contrôle de la résistance aux antibiotiques (866)
Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
(ISO format)
FRANCOIS, Patrice et al. Rapid and high-throughput genotyping of Staphylococcus epidermidis isolates by automated multilocus variable-number of tandem repeats: a tool for real-time epidemiology. In: Journal of microbiological methods, 2008, vol. 72, n° 3, p. 296-305. doi: 10.1016/j.mimet.2007.12.007 https://archive-ouverte.unige.ch/unige:853

540 hits



Deposited on : 2009-02-18

Export document
Format :
Citation style :