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Title

Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
Authors
Holtzer, Laurentius Henricus
Oleinich, Igor
Anzola, Marcello
Lindberg, Eric
Sadhu, Kalyan Kumar
Gonzalez Gaitan, Marcos
Winssinger, Nicolas
Published in ACS Central Science. 2016, vol. 2, no. 6, p. 394-400
Abstract Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.
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Article (Published version) (5.7 MB) - public document Free access
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Faculté des sciences / Section de chimie et biochimie / Département de chimie organique
Faculté des sciences / Section de chimie et biochimie / Département de biochimie
Research groups Groupe Gonzalez Gaitan
Groupe Winssinger
Citation
(ISO format) 		HOLTZER, Laurentius Henricus et al. Nucleic Acid Templated Chemical Reaction in a Live Vertebrate. In: ACS Central Science, 2016, vol. 2, n° 6, p. 394-400. doi: 10.1021/acscentsci.6b00054 https://archive-ouverte.unige.ch/unige:84737

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Deposited on : 2016-06-27

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