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Nucleic Acid Templated Chemical Reaction in a Live Vertebrate

Published in ACS central science. 2016, vol. 2, no. 6, p. 394-400
Abstract Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.
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Research groups Groupe Gonzalez Gaitan
Groupe Winssinger
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HOLTZER, Laurentius Henricus et al. Nucleic Acid Templated Chemical Reaction in a Live Vertebrate. In: ACS central science, 2016, vol. 2, n° 6, p. 394-400. doi: 10.1021/acscentsci.6b00054 https://archive-ouverte.unige.ch/unige:84737

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Deposited on : 2016-06-27

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