Nucleic Acid Templated Chemical Reaction in a Live Vertebrate

Article Article scientifique Article Nucleic Acid Templated Chemical Reaction in a Live Vertebrate eng 2016 author Holtzer Laurentius Henricus 722865 author Oleinich Igor 829964 author Anzola Marcello 810730 author Lindberg Eric 827425 author Sadhu Kalyan Kumar 811588 author Gonzalez Gaitan Marcos 645929 author Winssinger Nicolas 769664 394-400 10.1021/acscentsci.6b00054 2374-7943 Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate. Dewey 540 36 Département de chimie organique 141 39 Département de biochimie 144 false 765 Groupe Gonzalez Gaitan 762 Groupe Winssinger ACS central science 2 6 44c58510-85a1-451a-a6ed-995c9e24133c Article (Published version) Public acscentsci%2E6b00054.pdf 5973726 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie organique Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Nucleic Acid Templated Chemical Reaction in a Live Vertebrate	ACS central science			2016	685	291

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Oleinich, Igor