Cell rearrangement in transplanted human islets

Lavallard, Vanessa; Armanet, Mathieu; Parnaud, Géraldine; Meyer, Jérémy; Barbieux, Charlotte; Montanari, Elisa; Meier, Raphaël; Morel, Philippe; Berney, Thierry; Bosco, Domenico

doi:10.1096/fj.15-273805

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Title		Cell rearrangement in transplanted human islets
Authors		Lavallard, Vanessa Armanet, Mathieu Parnaud, Géraldine Meyer, Jérémy Barbieux, Charlotte Montanari, Elisa Meier, Raphaël Morel, Philippe Berney, Thierry Bosco, Domenico show all authors [1 - 10]
Published in		FASEB Journal. 2016, vol. 30, no. 2, p. 748-60
Abstract		The major feature of the human pancreatic islet architecture is the organization of endocrine cells into clusters comprising central β cells and peripheral α cells surrounded by vasculature. To have an insight into the mechanisms that govern this unique islet architecture, islet cells were isolated, and reaggregation of α and β cells into islet-like structures (pseudoislets) after culture or transplantation into mice was studied by immunohistology. The pseudoislets formed in culture displayed an unusual cell arrangement, contrasting with the transplanted pseudoislets, which exhibited a cell arrangement similar to that observed in native pancreatic islet subunits. The pattern of revascularization and the distribution of extracellular matrix around transplanted pseudoislets were alike to those observed in native pancreatic islets. This organization of transplanted pseudoislets occurred also when revascularization was abolished by treating mice with an anti-VEGF antibody, but not when contact with extracellular matrix was prevented by encapsulation of pseudoislets within alginate hydrogel. These results indicate that the maintenance of islet cell arrangement is dependent on in vivo features such as extracellular matrix but independent of vascularization.-Lavallard, V., Armanet, M., Parnaud, G., Meyer, J., Barbieux, C., Montanari, E., Meier, R., Morel, P., Berney, T., Bosco, D. Cell rearrangement in transplanted human islets.
Keywords		Architecture — Vascularization — Extracellular matrix
Identifiers		DOI: 10.1096/fj.15-273805 PMID: 26534832
Full text		Article (Published version) (3.2 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie
Research group		La transplantation d'îlots de Langerhans (623)
Citation (ISO format)		LAVALLARD, Vanessa et al. Cell rearrangement in transplanted human islets. In: FASEB Journal, 2016, vol. 30, n° 2, p. 748-60. doi: 10.1096/fj.15-273805 https://archive-ouverte.unige.ch/unige:84226