Experimental design for enantioselective separation of celiprolol by capillary electrophoresis using sulfated beta-cyclodextrin

Daali, Youssef; Cherkaoui, Samir; Christen, Philippe; Veuthey, Jean-Luc

doi:10.1002/(SICI)1522-2683(19991101)20:17<3424::AID-ELPS3424>3.0.CO;2-Z

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Title		Experimental design for enantioselective separation of celiprolol by capillary electrophoresis using sulfated beta-cyclodextrin
Authors		Daali, Youssef Cherkaoui, Samir Christen, Philippe Veuthey, Jean-Luc
Published in		Electrophoresis. 1999, vol. 20, no. 17, p. 3424-31
Abstract		A capillary zone electrophoresis method was developed for the enantioseparation of celiprolol enantiomers, using a sulfated beta-cyclodextrin (beta-CD) as a chiral selector. The use of a coated capillary was necessary to achieve stable and reproducible enantioseparations. A central composite design was applied to optimize the method and four parameters were selected for this study: the buffer pH, the buffer concentration, the sulfated beta-CD concentration and the temperature. Resolution between celiprolol enantiomers as well as analysis time and generated current were established as responses. For each response, a model was obtained by a second-degree mathematical expression. From the models, the most favorable conditions were determined by optimizing the resolution between celiprolol enantiomers and by setting the two other responses at threshold values. Response surfaces were also used to assess the robustness of the analytical method around the optimal region. Successful results were obtained with a 52 mM acetate buffer at pH 4.0 in the presence of 3.0 mM sulfated beta-CD at a temperature of 19.5 degrees C. Under these optimized conditions, baseline separation of the celiprolol enantiomers was achieved in less than 10 min. The method showed good validation data in terms of precision, accuracy and linearity, and was found to be suitable in determining celiprolol enantiomers in pharmaceutical preparations and in biological fluids.
Keywords		Adrenergic beta-Antagonists/isolation & purification/urine — Celiprolol/isolation & purification/urine — Cyclodextrins/chemistry — Electrophoresis, Capillary/methods — Humans — Reproducibility of Results — Stereoisomerism — Sulfates/chemistry — Tablets/chemistry — Beta-Cyclodextrins
Identifiers		DOI: 10.1002/(SICI)1522-2683(19991101)20:17<3424::AID-ELPS3424>3.0.CO;2-Z PMID: 10608710
Full text		Article (Published version) (1.1 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences Faculté des sciences / Section des sciences pharmaceutiques
Research group		Pharmaco-omiques et médecine de précision (1003)
Citation (ISO format)		DAALI, Youssef et al. Experimental design for enantioselective separation of celiprolol by capillary electrophoresis using sulfated beta-cyclodextrin. In: Electrophoresis, 1999, vol. 20, n° 17, p. 3424-31. doi: 10.1002/(SICI)1522-2683(19991101)20:17<3424::AID-ELPS3424>3.0.CO;2-Z https://archive-ouverte.unige.ch/unige:8414