en
Scientific article
English

Development of validated stereoselective methods for methadone determination in clinical samples

Published inChirality, vol. 11, no. 5-6, p. 487-494
Publication date1999
Abstract

A stereoselective analysis of methadone (Mtd) in whole blood and serum was developed using liquid chromatography on a protein based chiral stationary phase. Liquid-liquid extraction (LLE) and solid phase extraction methods were applied before chromatographic analysis. The extraction procedure, as well as the choice of the biological matrix, showed significant differences in the extraction yield and in the precision of the assays. Serum was selected for this assay and LLE was chosen as the preparation step because of its simplicity and rapidity. The total procedure was validated and applied to clinical samples. Samples taken from 45 heroin-addicted patients were analyzed. A correlation was found between the dose administered and Mtd concentration (total and R-form), but interindividual variability of the total normalized Mtd was seen (concentration varied from 90 to 530 ng/ml). Furthermore, two populations were apparently observed with a mean Mtd concentration of 200 and 475 ng/ml, respectively. Stereoselective analyses showed that more than 50% of the patients presented a nonracemic ratio, and particularly about 25% showed a preferential metabolism of the active R-Mtd enantiomer. Therefore, the stereoselective determination of Mtd is necessary to improve the quality of the treatment of heroin addiction.

Keywords
  • Chromatography, Liquid/methods
  • Heroin Dependence/rehabilitation
  • Humans
  • Methadone/blood/isolation & purification
  • Stereoisomerism
Citation (ISO format)
RUDAZ, Serge et al. Development of validated stereoselective methods for methadone determination in clinical samples. In: Chirality, 1999, vol. 11, n° 5-6, p. 487–494. doi: 10.1002/(SICI)1520-636X(1999)11:5/6<487::AID-CHIR22>3.0.CO;2-3
Main files (1)
Article (Published version)
accessLevelRestricted
ISSN of the journal0899-0042
559views
0downloads

Technical informations

Creation2010/06/22 14:05:00
First validation2010/06/22 14:05:00
Update time2023/03/14 15:49:11
Status update2023/03/14 15:49:11
Last indexation2024/01/15 20:32:44
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack