UNIGE document Scientific Article
previous document  unige:773  next document
add to browser collection
Title

Glial responses to neonatal hypoxic-ischemic injury in the rat cerebral cortex

Authors
Camm, Emily J.
Published in International Journal of Developmental Neuroscience. 2008, vol. 26, no. 1, p. 37-45
Abstract Neurogenesis is nearly completed after birth, whereas gliogenic activities remain intense during the postnatal period in the developing rat cortex. These include involution of radial glia, proliferation of astrocytes and oligodendrocytes and myelin formation. Little is known about the effects of hypoxic-ischemic (HI) injury on these critical postnatal processes. Here we explored the glial reactions to mild HI injury of the neonatal rat cerebral cortex at P3. We show that the HI lesion results in disruption of the normal radial glia architecture, which was paralleled by an increase in GFAP immunopositive reactive astrocytes. The morphology of these latter cells and the fact that they were immunolabelled for both nestin and GFAP suggest an accelerated transformation of radial glia into astrocytes. In addition, BrdU/GFAP immunostaining revealed a significant increase of double-labelled cells indicating an acute proliferation of astrocytes after HI. This enhanced proliferative activity of astrocytes persisted for several weeks. We found an elevated number and increased mitotic activity of both NG2-positive oligodendrocyte progenitors and RIP-positive oligodendrocytes after injury. These findings imply that glial responses are central to cortical tissue remodelling following neonatal ischemia and represent a potential target for therapeutic approaches.
Keywords AnimalsAnimals NewbornBromodeoxyuridine/metabolismCell Count/methodsCell Death/physiologyCerebral Cortex/pathologyDisease Models AnimalGene Expression Regulation Developmental/physiologyHypoxia-Ischemia Brain/pathologyIn Situ Nick-End Labeling/methodsNerve Tissue Proteins/metabolismNeuroglia/metabolism/pathologyRatsRats WistarStilbamidines/metabolismTime Factors
Identifiers
PMID: 17942266
Full text
Structures
Research groups Progéniteurs neuronaux (141)
Groupe Dayer Alexandre (Formation du circuit cortical) (875)
Citation
(ISO format)
SIZONENKO, Stéphane et al. Glial responses to neonatal hypoxic-ischemic injury in the rat cerebral cortex. In: International Journal of Developmental Neuroscience, 2008, vol. 26, n° 1, p. 37-45. https://archive-ouverte.unige.ch/unige:773

302 hits

1 download

Update

Deposited on : 2009-02-12

Export document
Format :
Citation style :