Scientific article

Comparative efficacy of daptomycin and vancomycin in the therapy of experimental foreign body infection due to Staphylococcus aureus

Published inThe journal of antimicrobial chemotherapy, vol. 52, no. 1, p. 89-95
Publication date2003

The therapeutic activity of daptomycin was compared with that of vancomycin in a rat model of subcutaneously implanted tissue cages chronically infected with strain Rev1, a spontaneous methicillin-susceptible revertant of the methicillin-resistant Staphylococcus aureus strain MRGR3, showing equivalent virulence to its parent. The MIC and MBC of daptomycin (in Mueller-Hinton broth supplemented with 50 mg/L Ca2+) or vancomycin for strain Rev1 were 1-2 and 2-4 or 1 and 2 mg/L, respectively. In vitro elimination of strain Rev1 in the presence of 50% tissue cage fluid was more rapid with daptomycin 4 mg/L compared with vancomycin. After 2 weeks of infection, viable counts of strain Rev1 averaged 6.49 log10 cfu/mL of tissue cage fluid (n = 87). Intraperitoneal administration of daptomycin 30 mg/kg once daily, or vancomycin 50 mg/kg twice daily, produced antibiotic levels continuously above MBC. After 7 days of therapy with daptomycin or vancomycin, mean +/- S.E.M. counts of Rev1 decreased (P < 0.05) by 1.11 +/- 0.25 (n = 28) or 0.80 +/- 0.31 (n = 35) log10 cfu/mL, respectively, compared with cages of untreated animals, but were not significantly different from each other. In daptomycin-treated rats, three cages yielded subpopulations with reduced susceptibility to daptomycin. In conclusion, a low dose regimen of daptomycin was at least equivalent to vancomycin against chronic foreign body infections due to S. aureus. Drug dosage should be adapted to obtain inflammatory fluid levels of daptomycin minimizing emergence of resistant subpopulations.

  • Animals
  • Anti-Bacterial Agents/ therapeutic use
  • Area Under Curve
  • Daptomycin/ therapeutic use
  • Diffusion Chambers, Culture
  • Foreign Bodies
  • Kinetics
  • Methicillin Resistance
  • Microbial Sensitivity Tests
  • Rats
  • Staphylococcal Infections/ drug therapy/microbiology
  • Staphylococcus aureus/drug effects
  • Vancomycin/ therapeutic use
Citation (ISO format)
VAUDAUX, Pierre et al. Comparative efficacy of daptomycin and vancomycin in the therapy of experimental foreign body infection due to Staphylococcus aureus. In: The journal of antimicrobial chemotherapy, 2003, vol. 52, n° 1, p. 89–95. doi: 10.1093/jac/dkg277
Updates (1)
ISSN of the journal0305-7453

Technical informations

Creation06/21/2010 10:26:52 AM
First validation06/21/2010 10:26:52 AM
Update time03/14/2023 3:45:58 PM
Status update03/14/2023 3:45:58 PM
Last indexation08/28/2023 6:21:33 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack