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Scientific article
English

Intracellular pattern of cytosolic Ca2+ changes during adhesion and multiple phagocytosis in human neutrophils. Dynamics of intracellular Ca2+ stores

Contributors

Theler, J. M.; Lew, Daniel Pablo; Jaconi, M. E.; Krause, Karl-Heinz; Wollheim, Claes; Schlegel, Werner

Published in

Blood, vol. 85, no. 8, p. 2194-2201

Publication date

1995

Abstract

The subcellular pattern of cytosolic free Ca2+ ([Ca2+]i) changes in human polymorphonuclear neutrophils (PMNs) was studied using imaging of fura-2 fluorescence (time resolution 12.5 ratios/s) to determine whether PMNs could obtain directional information from the [Ca2+]i signal. [Ca2+]i changes were observed during initial adherence, the subsequent chemotactic movement, and the phagocytosis of opsonized yeast particles. Initial adherence was followed by a rapid increase in [Ca2+]i (from 90 +/- 10 to 290 +/- 40 nmol/L in 6.5 +/- 2.5 seconds; +/- SEM, n = 10), apparently homogeneously distributed over the entire cytoplasm, which preceded the spreading of the PMNs. [Ca2+]i increases after the contact of the PMNs with yeast particles were of lower mean amplitude; [Ca2+]i increased simultaneously throughout the cytosol. In the absence of extracellular Ca2+, multiple phagocytotic events could proceed normally without a mandatory [Ca2+]i transient. In PMNs polarized on phagocytosis, gradients in [Ca2+]i could be observed. [Ca2+]i was more elevated in the periphagosomal area than in the remaining parts. Taken together, these data show that [Ca2+]i waves do not provide the neutrophil with directional information during chemotaxis and phagocytosis. Sustained small inhomogeneity of [Ca2+]i levels are consistent with a proposed redistribution of releasable Ca2+ stores on phagocytosis.

Keywords

  • Adult
  • Calcium/ metabolism
  • Cell Adhesion
  • Cell Compartmentation
  • Chemotaxis, Leukocyte
  • Complement System Proteins
  • Cytosol/chemistry
  • Humans
  • Image Processing, Computer-Assisted
  • Intracellular Fluid/chemistry
  • Neutrophils/chemistry/ physiology/ultrastructure
  • Phagocytosis
  • Saccharomyces cerevisiae
  • Videotape Recording

UNIGE affiliation entities

Citation (ISO format)

THELER, J. M. et al. Intracellular pattern of cytosolic Ca2+ changes during adhesion and multiple phagocytosis in human neutrophils. Dynamics of intracellular Ca2+ stores. In: Blood, 1995, vol. 85, n° 8, p. 2194–2201.

Main files (1)

Article

Identifiers

Additional URL for this publication

http://bloodjournal.hematologylibrary.org/cgi/reprint/85/8/2194.pdf

Journal ISSN

0006-4971
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Technical informations

Creation

21/06/2010 10:26:35

First validation

21/06/2010 10:26:35

Update

14/03/2023 15:45:44

Status update

14/03/2023 15:45:44

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