Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes

Lukowski, Samuel; Fish, Richard; Martin-Levilain, Juliette; Gonelle-Gispert, Carmen; Buehler, Leo Hans; Maechler, Pierre; Dermitzakis, Emmanouil; Neerman Arbez, Marguerite

doi:10.1016/j.ygeno.2015.05.001

Scientific article

English

Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes

Contributors

Lukowski, Samuel; Fish, Richard

; Martin-Levilain, Juliette; Gonelle-Gispert, Carmen; Buehler, Leo Hans; Maechler, Pierre

; Dermitzakis, Emmanouil; Neerman Arbez, Marguerite

Published in

Genomics, vol. 106, no. 2, p. 107-115

Publication date

2015

Abstract

The expression of plasma proteins changes dramatically as a result of cytokine induction, particularly interleukin-6, and their levels are used as clinical markers of inflammation. miRNAs are important regulators of gene expression and play significant roles in many inflammatory diseases and processes. The interactions between miRNAs and the genes that they regulate during the acute phase response have not been investigated. We examined the effects of IL-6 stimulation on the transcriptome and miRNome of human and mouse primary hepatocytes and the HepG2 cell line. Using an integrated analysis, we identified differentially expressed miRNAs whose seed sequences are significantly enriched in the 3' untranslated regions of differentially expressed genes, many of which are involved in inflammation-related pathways. Our finding that certain miRNAs may de-repress critical acute phase proteins within acute timeframes has important biological and clinical implications.

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

LUKOWSKI, Samuel et al. Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes. In: Genomics, 2015, vol. 106, n° 2, p. 107–115. doi: 10.1016/j.ygeno.2015.05.001

Article (Accepted version)

Identifiers

PID : unige:75016
DOI : 10.1016/j.ygeno.2015.05.001
PMID : 25979460

Journal ISSN

0888-7543

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03/09/2015 16:20:00

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03/09/2015 16:20:00

Update

14/03/2023 23:35:51

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14/03/2023 23:35:50

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16/12/2024 14:52:44

Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

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Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation