Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes

Lukowski, Samuel; Fish, Richard; Martin-Levilain, Juliette; Gonelle-Gispert, Carmen; Buehler, Leo Hans; Maechler, Pierre; Dermitzakis, Emmanouil; Neerman Arbez, Marguerite

doi:10.1016/j.ygeno.2015.05.001

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Title		Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes
Authors		Lukowski, Samuel Fish, Richard Martin-Levilain, Juliette Gonelle-Gispert, Carmen Buehler, Leo Hans Maechler, Pierre Dermitzakis, Emmanouil Neerman Arbez, Marguerite
Published in		Genomics. 2015, vol. 106, no. 2, p. 107-115
Abstract		The expression of plasma proteins changes dramatically as a result of cytokine induction, particularly interleukin-6, and their levels are used as clinical markers of inflammation. miRNAs are important regulators of gene expression and play significant roles in many inflammatory diseases and processes. The interactions between miRNAs and the genes that they regulate during the acute phase response have not been investigated. We examined the effects of IL-6 stimulation on the transcriptome and miRNome of human and mouse primary hepatocytes and the HepG2 cell line. Using an integrated analysis, we identified differentially expressed miRNAs whose seed sequences are significantly enriched in the 3' untranslated regions of differentially expressed genes, many of which are involved in inflammation-related pathways. Our finding that certain miRNAs may de-repress critical acute phase proteins within acute timeframes has important biological and clinical implications.
Identifiers		DOI: 10.1016/j.ygeno.2015.05.001 PMID: 25979460
Full text		Article (Accepted version) (3.4 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement
Research groups		Recherche translationnelle : anomalies de l'hémostase et thromboses (504) Population Genomics and Genetics of Complex Traits (892)
Citation (ISO format)		LUKOWSKI, Samuel et al. Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes. In: Genomics, 2015, vol. 106, n° 2, p. 107-115. doi: 10.1016/j.ygeno.2015.05.001 https://archive-ouverte.unige.ch/unige:75016