UNIGE document Scientific Article
previous document  unige:74428  next document
add to browser collection
Title

Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection

Authors
Lobrinus, Johannes-Alexander
show hidden authors show all authors [1 - 15]
Published in Biomaterials. 2015, vol. 53, p. 296-308
Abstract Human cytomegalovirus (HCMV) is the most common cause of congenital infection of the central nervous system (CNS). To overcome the limited access to human neural tissue and stringent species specificity of HCMV, we used engineered neural tissues to: (i) provide a technical advance to mimick features of HCMV infection in a human neural fetal tissue in vitro and (ii) characterize the molecular and cellular phenomenon following HCMV infection in this tissue. Herein, we infected hESC-derived engineered neural tissues (ENTs) whose organization resembles fetal brain. Transcriptome analysis of ENTs demonstrated that HCMV infection displayed features of the infection with the expression of genes involved in lipid metabolism, growth and development, as well as stress and host-response in a time-dependent manner. Immunohistochemical analysis demonstrated that HCMV did not firstly infect neural tubes (i.e. radially organized, proliferating stem cell niches), but rather an adjacent side population of post-mitotic cells expressing nestin, doublecortin, Sox1, musashi and vimentin markers. Importantly, we observe the same tropism in naturally HCMV-infected fetal brain specimens. To the best of our knowledge this system represents the first human brain-like tissue able to provide a more physiologically model for studying HCMV infection.
Identifiers
PMID: 25890728
Full text
Article (Published version) (5.5 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Groupe Laurent Kaiser (virologie clinique) (668)
Identification des facteurs clés impliqués dans la pathogénèse des rhinovirus et entérovirus (953)
Radicaux libres et cellules souches embryonnaires (60)
Métastases du foie (657)
Groupe Desmeules Jules (pharmacologie/toxicologie) (567)
Citation
(ISO format)
COSSET, Erika et al. Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection. In: Biomaterials, 2015, vol. 53, p. 296-308. https://archive-ouverte.unige.ch/unige:74428

362 hits

8 downloads

Update

Deposited on : 2015-07-29

Export document
Format :
Citation style :