Article (Published version) (411 Kb) - Free access
Fulminant atypical Cryptococcus neoformans pneumonia confirmed by PLEX-ID.
|Published in||International Journal of Infectious Diseases. 2014, vol. 22, p. 17-8|
|Abstract||Use of the PLEX-ID system can lead to a rapid molecular diagnosis in microbiology. To illustrate the clinical implications of this new diagnostic tool, we present the case of a 46-year-old patient admitted with severe respiratory failure and septic shock. Cryptococcal pneumonia was diagnosed by Fungi-Fluor™ staining of the bronchoalveolar lavage (BAL) and the patient tested positive for HIV. Unfortunately, he died 12h after admission despite intensive care support and treatment with broad-spectrum antibiotics, amphotericin B, and flucytosine. Retrospective use of the PLEX-ID on the BAL, bronchial aspirate, and blood yielded Cryptococcus neoformans in all fluids tested. Rapid molecular diagnosis with PLEX-ID, especially when performed on the blood of septic patients, may reduce the time to adequate treatment and limit the number of diagnostic procedures needed.|
|Keywords||Amphotericin B/therapeutic use — Anti-Bacterial Agents/therapeutic use — Antifungal Agents/therapeutic use — Bronchoalveolar Lavage Fluid/microbiology — Coinfection — Cryptococcosis/diagnosis/drug therapy/microbiology/pathology — Cryptococcus neoformans/genetics/isolation & purification — Fatal Outcome — Female — Flucytosine/therapeutic use — HIV Infections/diagnosis/pathology/virology — Humans — Male — Middle Aged — Nucleic Acid Amplification Techniques — Pneumonia/diagnosis/drug therapy/microbiology/pathology|
|Research group||Analyse génomique et fonctionnelle du staphylocoque doré (604)|
|GARIANI, Karim et al. Fulminant atypical Cryptococcus neoformans pneumonia confirmed by PLEX-ID. In: International Journal of Infectious Diseases, 2014, vol. 22, p. 17-8. https://archive-ouverte.unige.ch/unige:74112|