en
Scientific article
Open access
English

Characterization of Streptococcus tigurinus small-colony variants causing prosthetic joint infection by comparative whole-genome analyses

Published inJournal of clinical microbiology, vol. 52, no. 2, p. 467-474
Publication date2014
Abstract

Small-colony variants (SCVs) of bacteria are associated with recurrent and persistent infections. We describe for the first time SCVs of Streptococcus tigurinus in a patient with a prosthetic joint infection. S. tigurinus is a novel pathogen of the Streptococcus mitis group and causes invasive infections. We sought to characterize S. tigurinus SCVs using experimental methods and find possible genetic explanations for their phenotypes. The S. tigurinus SCVs were compared with the wild-type (WT) isolate using phenotypic methods, including growth under different conditions, autolysis, and visualization of the cell ultrastructure by use of transmission electron microscopy (TEM). Furthermore, comparative genome analyses were performed. The S. tigurinus SCVs displayed reduced growth compared to the WT and showed either a very stable or a fluctuating SCV phenotype. TEM analyses revealed major alterations in cell separation and morphological abnormalities, which were partially explained by impaired autolytic behavior. Intriguingly, the SCVs were more resistant to induced autolysis. Whole-genome sequencing revealed mutations in the genes involved in general cell metabolism, cell division, stringent response, and virulence. Clinically, the patient recovered after a 2-stage exchange of the prosthesis. Comparative whole-genome sequencing in clinical strains is a useful tool for identifying novel genetic signatures leading to the most persistent bacterial forms. The detection of viridans streptococcal SCVs is challenging in a clinical laboratory due to the small colony size. Thus, it is of major clinical importance for microbiologists and clinicians to be aware of viridans streptococcal SCVs, such as those of S. tigurinus, which lead to difficult-to-treat infections.

Keywords
  • Aged, 80 and over
  • Arthritis/microbiology
  • DNA Mutational Analysis
  • DNA, Bacterial/chemistry/genetics
  • Female
  • Genome, Bacterial
  • Humans
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Mutation
  • Prosthesis-Related Infections/microbiology
  • Sequence Analysis, DNA
  • Streptococcal Infections/microbiology
  • Streptococcus/genetics/growth & development/isolation & purification/ultrastructure
Citation (ISO format)
ZBINDEN, Andrea et al. Characterization of Streptococcus tigurinus small-colony variants causing prosthetic joint infection by comparative whole-genome analyses. In: Journal of clinical microbiology, 2014, vol. 52, n° 2, p. 467–474. doi: 10.1128/JCM.02801-13
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0095-1137
593views
319downloads

Technical informations

Creation06/15/2015 2:21:00 PM
First validation06/15/2015 2:21:00 PM
Update time03/14/2023 11:27:43 PM
Status update03/14/2023 11:27:43 PM
Last indexation01/16/2024 6:32:16 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack