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Scientific article
English

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Published inJournal of clinical microbiology, vol. 42, no. 7, p. 3309-3312
Publication date2004
Abstract

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

Keywords
  • Bacterial Proteins/ genetics
  • Bacterial Typing Techniques
  • Chromosomes, Bacterial
  • Methicillin Resistance
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction/ methods
  • Staphylococcus aureus/classification/drug effects/ genetics
Citation (ISO format)
FRANCOIS, Patrice et al. A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. In: Journal of clinical microbiology, 2004, vol. 42, n° 7, p. 3309–3312. doi: 10.1128/JCM.42.7.3309-3312.2004
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ISSN of the journal0095-1137
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