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Title

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Authors
Bento, Manuela
Published in Journal of Clinical Microbiology. 2004, vol. 42, no. 7, p. 3309-3312
Abstract We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.
Keywords Bacterial Proteins/ geneticsBacterial Typing TechniquesChromosomes, BacterialMethicillin ResistanceMicrobial Sensitivity TestsPolymerase Chain Reaction/ methodsStaphylococcus aureus/classification/drug effects/ genetics
Identifiers
PMID: 15243102
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Structures
Research groups Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866)
Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
Citation
(ISO format)
FRANCOIS, Patrice et al. A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. In: Journal of Clinical Microbiology, 2004, vol. 42, n° 7, p. 3309-3312. https://archive-ouverte.unige.ch/unige:7229

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Deposited on : 2010-06-21

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