A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Francois, Patrice; Renzi, Gesuele; Pittet, Didier; Bento, Manuela; Lew, Daniel Pablo; Harbarth, Stéphan Juergen; Vaudaux, Pierre; Schrenzel, Jacques

doi:10.1128/JCM.42.7.3309-3312.2004

Scientific article

English

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Contributors

Francois, Patrice

; Renzi, Gesuele; Pittet, Didier; Bento, Manuela; Lew, Daniel Pablo; Harbarth, Stéphan Juergen

; Vaudaux, Pierre; Schrenzel, Jacques

Published in

Journal of clinical microbiology, vol. 42, no. 7, p. 3309-3312

Publication date

2004

Abstract

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

Keywords

Bacterial Proteins/ genetics
Bacterial Typing Techniques
Chromosomes, Bacterial
Methicillin Resistance
Microbial Sensitivity Tests
Polymerase Chain Reaction/ methods
Staphylococcus aureus/classification/drug effects/ genetics

UNIGE affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

UNIGE research groups

Citation (ISO format)

FRANCOIS, Patrice et al. A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. In: Journal of clinical microbiology, 2004, vol. 42, n° 7, p. 3309–3312. doi: 10.1128/JCM.42.7.3309-3312.2004

Article

Identifiers

PID : unige:7229
DOI : 10.1128/JCM.42.7.3309-3312.2004
PMID : 15243102

Additional URL for this publication

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC446242/pdf/0081-04.pdf

Journal ISSN

0095-1137

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21/06/2010 10:24:08

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10/11/2026 13:11:13

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

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Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation