Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

Francois, Patrice; Koessler, Thibaud; Huyghe, Antoine; Harbarth, Stéphan Juergen; Bento, Manuela; Lew, Daniel Pablo; Etienne, Jérôme; Pittet, Didier; Schrenzel, Jacques

doi:10.1128/JCM.44.5.1892-1895.2006

Scientific article

English

Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

Contributors

Francois, Patrice

; Koessler, Thibaud; Huyghe, Antoine; Harbarth, Stéphan Juergen

; Bento, Manuela; Lew, Daniel Pablo; Etienne, Jérôme; Pittet, Didier; Schrenzel, Jacques

Published in

Journal of clinical microbiology, vol. 44, no. 5, p. 1892-1895

Publication date

2006

Abstract

The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.

Keywords

Bacterial Proteins/ genetics
Bacterial Typing Techniques
Base Sequence
DNA Primers/genetics
DNA Probes/genetics
DNA, Bacterial/genetics/isolation & purification
Genes, Bacterial
Genotype
Humans
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction/ methods/statistics & numerical data
Staphylococcus aureus/ classification/ genetics/isolation & purification/pathogenicity
Trans-Activators/ genetics
Virulence/genetics

UNIGE affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

UNIGE research groups

Citation (ISO format)

FRANCOIS, Patrice et al. Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay. In: Journal of clinical microbiology, 2006, vol. 44, n° 5, p. 1892–1895. doi: 10.1128/JCM.44.5.1892-1895.2006

Article

Identifiers

PID : unige:7227
DOI : 10.1128/JCM.44.5.1892-1895.2006
PMID : 16672433

Additional URL for this publication

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479209/pdf/2172-05.pdf

Journal ISSN

0095-1137

707views

0downloads

Creation

21/06/2010 10:24:07

First validation

21/06/2010 10:24:07

Update

14/10/2025 06:58:07

Status update

14/03/2023 15:43:34

Last indexation

10/11/2026 13:11:13

Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation