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Title

Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

Authors
Koessler, Thibaud
Bento, Manuela
Etienne, Jérôme
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Published in Journal of Clinical Microbiology. 2006, vol. 44, no. 5, p. 1892-1895
Abstract The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.
Keywords Bacterial Proteins/ geneticsBacterial Typing TechniquesBase SequenceDNA Primers/geneticsDNA Probes/geneticsDNA, Bacterial/genetics/isolation & purificationGenes, BacterialGenotypeHumansMolecular Sequence DataPhylogenyPolymerase Chain Reaction/ methods/statistics & numerical dataStaphylococcus aureus/ classification/ genetics/isolation & purification/pathogenicityTrans-Activators/ geneticsVirulence/genetics
Identifiers
PMID: 16672433
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Research groups Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866)
Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
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FRANCOIS, Patrice et al. Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay. In: Journal of Clinical Microbiology, 2006, vol. 44, n° 5, p. 1892-1895. https://archive-ouverte.unige.ch/unige:7227

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Deposited on : 2010-06-21

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