Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

Francois, Patrice; Koessler, Thibaud; Huyghe, Antoine; Harbarth, Stéphan Juergen; Bento, Manuela; Lew, Daniel Pablo; Etienne, Jérôme; Pittet, Didier; Schrenzel, Jacques

doi:10.1128/JCM.44.5.1892-1895.2006

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Title		Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay
Authors		Francois, Patrice Koessler, Thibaud Huyghe, Antoine Harbarth, Stéphan Juergen Bento, Manuela Lew, Daniel Pablo Etienne, Jérôme Pittet, Didier Schrenzel, Jacques show all authors [1 - 9]
Published in		Journal of Clinical Microbiology. 2006, vol. 44, no. 5, p. 1892-1895
Abstract		The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.
Keywords		Bacterial Proteins/ genetics — Bacterial Typing Techniques — Base Sequence — DNA Primers/genetics — DNA Probes/genetics — DNA, Bacterial/genetics/isolation & purification — Genes, Bacterial — Genotype — Humans — Molecular Sequence Data — Phylogeny — Polymerase Chain Reaction/ methods/statistics & numerical data — Staphylococcus aureus/ classification/ genetics/isolation & purification/pathogenicity — Trans-Activators/ genetics — Virulence/genetics
Identifiers		DOI: 10.1128/JCM.44.5.1892-1895.2006 PMID: 16672433
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479209/pdf/2172-05.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research groups		Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866) Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
Citation (ISO format)		FRANCOIS, Patrice et al. Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay. In: Journal of Clinical Microbiology, 2006, vol. 44, n° 5, p. 1892-1895. doi: 10.1128/JCM.44.5.1892-1895.2006 https://archive-ouverte.unige.ch/unige:7227