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Scientific article
English

Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

Published inJournal of clinical microbiology, vol. 44, no. 5, p. 1892-1895
Publication date2006
Abstract

The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.

Keywords
  • Bacterial Proteins/ genetics
  • Bacterial Typing Techniques
  • Base Sequence
  • DNA Primers/genetics
  • DNA Probes/genetics
  • DNA, Bacterial/genetics/isolation & purification
  • Genes, Bacterial
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction/ methods/statistics & numerical data
  • Staphylococcus aureus/ classification/ genetics/isolation & purification/pathogenicity
  • Trans-Activators/ genetics
  • Virulence/genetics
Citation (ISO format)
FRANCOIS, Patrice et al. Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay. In: Journal of clinical microbiology, 2006, vol. 44, n° 5, p. 1892–1895. doi: 10.1128/JCM.44.5.1892-1895.2006
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ISSN of the journal0095-1137
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