Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus

Francois, Patrice; Bento, Manuela; Renzi, Gesuele; Harbarth, Stéphan Juergen; Pittet, Didier; Schrenzel, Jacques

doi:10.1128/JCM.00232-07

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Title		Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus
Authors		Francois, Patrice Bento, Manuela Renzi, Gesuele Harbarth, Stéphan Juergen Pittet, Didier Schrenzel, Jacques
Published in		Journal of Clinical Microbiology. 2007, vol. 45, no. 6, p. 2011-2013
Abstract		One home-developed assay and two commercial assays for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of clinical isolates displaying highly diverse genetic backgrounds. Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones.
Keywords		Bacterial Proteins/ genetics — Bacterial Typing Techniques — DNA, Bacterial/analysis/isolation & purification — Humans — Methicillin Resistance — Nucleic Acid Hybridization/ methods — Polymerase Chain Reaction/ methods — Reagent Kits, Diagnostic — Staphylococcal Infections/diagnosis/epidemiology/ microbiology — Staphylococcus aureus/ classification/ drug effects/genetics — Time Factors — Transcription Factors/ genetics
Identifiers		DOI: 10.1128/JCM.00232-07 PMID: 17428926
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933053/pdf/0232-07.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research groups		Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866) Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
Citation (ISO format)		FRANCOIS, Patrice et al. Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus. In: Journal of Clinical Microbiology, 2007, vol. 45, n° 6, p. 2011-2013. doi: 10.1128/JCM.00232-07 https://archive-ouverte.unige.ch/unige:7224