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Scientific article
English

Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus

Published inJournal of clinical microbiology, vol. 45, no. 6, p. 2011-2013
Publication date2007
Abstract

One home-developed assay and two commercial assays for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of clinical isolates displaying highly diverse genetic backgrounds. Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones.

Keywords
  • Bacterial Proteins/ genetics
  • Bacterial Typing Techniques
  • DNA, Bacterial/analysis/isolation & purification
  • Humans
  • Methicillin Resistance
  • Nucleic Acid Hybridization/ methods
  • Polymerase Chain Reaction/ methods
  • Reagent Kits, Diagnostic
  • Staphylococcal Infections/diagnosis/epidemiology/ microbiology
  • Staphylococcus aureus/ classification/ drug effects/genetics
  • Time Factors
  • Transcription Factors/ genetics
Citation (ISO format)
FRANCOIS, Patrice et al. Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus. In: Journal of clinical microbiology, 2007, vol. 45, n° 6, p. 2011–2013. doi: 10.1128/JCM.00232-07
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ISSN of the journal0095-1137
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