Development and validation of a new reversed-phase ion pairing liquid chromatographic method with fluorescence detection for penciclovir analysis in plasma and aqueous humor

Schenkel, F.; Rudaz, Serge; Daali, Youssef; Oestreicher, M Kondo; Veuthey, Jean-Luc; Dayer, Pierre

doi:10.1016/j.jchromb.2005.07.022

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Title		Development and validation of a new reversed-phase ion pairing liquid chromatographic method with fluorescence detection for penciclovir analysis in plasma and aqueous humor
Authors		Schenkel, F. Rudaz, Serge Daali, Youssef Oestreicher, M Kondo Veuthey, Jean-Luc Dayer, Pierre
Published in		Journal of chromatography. B. 2005, vol. 826, no. 1-2, p. 1-7
Abstract		A simple, sensitive and reliable HPLC ion-pairing method with fluorescence detection, was developed for penciclovir determination in plasma and aqueous humor, with a Zorbax SB-aq C18 (100 mmx2.1 mm) column. Plasma samples were treated by solid-phase extraction with Oasis MCX (30 mg) cartridges. Ganciclovir, an antiviral drug structurally related to penciclovir, was used as internal standard (I.S.). Aqueous humor samples were directly injected into the chromatographic system. Separation was performed by a gradient elution with a mobile phase consisting of a mixture of acetonitrile and phosphate buffer 50mM containing 5mM of sodium octanesulfonate, pH 2.0, at a flow rate of 0.3 ml/min. The method was validated and showed good performances in terms of linearity, sensitivity, precision and trueness. Quantification limit was obtained at 0.05 microg/ml for aqueous humor and at 0.1 microg/ml for plasma. Finally, the proposed analytical method was used to measure penciclovir in clinical samples for a pharmacokinetic study, after oral administration of famciclovir.
Keywords		Acyclovir/analogs & derivatives/analysis/blood — Aqueous Humor/chemistry — Chromatography, High Pressure Liquid/methods — Drug Stability — Ganciclovir/analysis — Humans — Hydrogen-Ion Concentration — Reproducibility of Results — Sensitivity and Specificity — Spectrometry, Fluorescence — Temperature
Identifiers		DOI: 10.1016/j.jchromb.2005.07.022 PMID: 16140045
Full text		Article (Published version) (230 Kb) - Limited access to UNIGE
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Pharmaco-omiques et médecine de précision (1003)
Citation (ISO format)		SCHENKEL, F. et al. Development and validation of a new reversed-phase ion pairing liquid chromatographic method with fluorescence detection for penciclovir analysis in plasma and aqueous humor. In: Journal of chromatography. B, 2005, vol. 826, n° 1-2, p. 1-7. doi: 10.1016/j.jchromb.2005.07.022 https://archive-ouverte.unige.ch/unige:6536