Scientific article
English

Optimized liquid chromatography-mass spectrometry approach for the isolation of minor stress biomarkers in plant extracts and their identification by capillary nuclear magnetic resonance

Published inJournal of chromatography, vol. 1180, p. 90-98
Publication date2008
Abstract

A LC-MS approach is presented for the isolation of minor key plant biomarkers, in view of their characterization by NMR at the microgram scale. Due to the complexity of plant extracts, the purification of metabolites present in low concentrations is critical. The strategy used relies on the optimization of the chromatographic analysis using ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS), thanks to modelling software. The optimized method is then transferred to semi-preparative LC conditions with MS detection. The approach is illustrated by the isolation of wound-induced jasmonate derivatives revealed by a metabolomic study in Arabidopsis thaliana leaves and their subsequent characterization by capillary NMR (CapNMR).

Keywords
  • Arabidopsis/*chemistry
  • Biological Markers/*analysis
  • Chromatography, High Pressure Liquid/*methods
  • Magnetic Resonance Spectroscopy/*methods
  • Plant Extracts/*chemistry
  • Spectrometry, Mass, Electrospray Ionization/*methods
Citation (ISO format)
GLAUSER, Gaétan et al. Optimized liquid chromatography-mass spectrometry approach for the isolation of minor stress biomarkers in plant extracts and their identification by capillary nuclear magnetic resonance. In: Journal of chromatography, 2008, vol. 1180, p. 90–98. doi: 10.1016/j.chroma.2007.12.021
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Journal ISSN0021-9673
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Creation29/10/2008 11:37:27
First validation29/10/2008 11:37:27
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