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Scientific article
English

High-throughput log P determination by MEEKC coupled with UV and MS detections

Published inElectrophoresis, vol. 31, no. 5, p. 952-964
Publication date2010
Abstract

A high-throughput screening method using MEEKC was developed for the determination of 1-octanol-water partition coefficients (log P(oct)). Two approaches were carried out to decrease determination times to about 20 min per compound: (i) a dynamically coated capillary was used to increase the EOF at low pH, allowing the measurement of log P(oct) of acidic compounds and (ii) a short-end injection was performed to reduce the capillary effective length. The analytical conditions were optimized to determine the lipophilicity of neutral, basic, and acidic compounds with log P(oct) ranging from 0 to 5. The developed method was first applied to a well-balanced set of 35 reference compounds, and second to a set of 21 acidic and 29 basic pharmaceutical compounds. Finally, determinations were achieved with MS detection, allowing a 20-fold throughput increase thanks to sample pooling. An atmospheric pressure photoionization source was selected to advantageously replace ESI as it was less affected by the non-volatile BGE additives used in MEEKC.

Keywords
  • CE-atmospheric pressure photoionization-MS
  • Dynamic coating
  • Lipophilicity
  • MEEKC
  • Partition coefficients
Citation (ISO format)
HENCHOZ, Yveline et al. High-throughput log P determination by MEEKC coupled with UV and MS detections. In: Electrophoresis, 2010, vol. 31, n° 5, p. 952–964. doi: 10.1002/elps.200900540
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ISSN of the journal0173-0835
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Creation03/11/2010 1:53:00 PM
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