Capillary electrophoresis and high-performance anion exchange chromatography for monitoring caseinoglycomacropeptide sialylation

Daali, Youssef; Cherkaoui, Samir; Veuthey, Jean-Luc

doi:10.1016/S0731-7085(00)00552-5

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Title		Capillary electrophoresis and high-performance anion exchange chromatography for monitoring caseinoglycomacropeptide sialylation
Authors		Daali, Youssef Cherkaoui, Samir Veuthey, Jean-Luc
Published in		Journal of pharmaceutical and biomedical analysis. 2001, vol. 24, no. 5-6, p. 849-56
Abstract		Capillary zone electrophoresis was applied to separate caseinoglycomacropeptide glycoforms and characterize microheterogeneity of the glycopeptide. Particular attention was paid to the sialic acid content in caseinoglycomacropeptide obtained through different manufacturing processes. A chemometric approach was used to simultaneously study effects of acid concentration, hydrolysis time and temperature on sialic acid release from caseinoglycomacropeptide. Hydrolysis conditions that maximize sialic acid release were chosen. Sialic acid was determined using high performance anion exchange chromatography coupled with pulsed amperometric detection. Results were compared to those obtained by alternative techniques, such as colorimetric and enzymatic methods.
Keywords		Anion Exchange Resins — Caseins/chemistry — Chromatography, High Pressure Liquid/methods — Chromatography, Ion Exchange/methods — Electrophoresis, Capillary/methods — Hydrolysis — N-Acetylneuraminic Acid/chemistry — Peptide Fragments/chemistry — Reproducibility of Results
Identifiers		DOI: 10.1016/S0731-7085(00)00552-5 PMID: 11248477
Full text		Article (Published version) (141 Kb) - Limited access to UNIGE
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Pharmaco-omiques et médecine de précision (1003)
Citation (ISO format)		DAALI, Youssef, CHERKAOUI, Samir, VEUTHEY, Jean-Luc. Capillary electrophoresis and high-performance anion exchange chromatography for monitoring caseinoglycomacropeptide sialylation. In: Journal of pharmaceutical and biomedical analysis, 2001, vol. 24, n° 5-6, p. 849-56. doi: 10.1016/S0731-7085(00)00552-5 https://archive-ouverte.unige.ch/unige:5139