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Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry

Chevalley, Anne-Françoise
Mino, Annie
Deglon, Jean-Jacques
Published in Journal of chromatography. 2000, vol. 871, no. 1-2, p. 163-72
Abstract Saliva was tested and evaluated as a biological matrix for methadone (Mtd) monitoring. Conventional method using a narrow bore C18 column, and an enantioselective method using a narrow bore alpha1-acid glycoprotein column, were developed using liquid chromatography coupled with a mass spectromeric (MS) detector. After optimisation of MS conditions by flow injection analysis, selected ion monitoring detection was used to enhance sensitivity. The total Mtd concentration and the enantiomeric ratio in saliva were validated using an experimental design. The methods were applied to samples provided by heroin addicts undergoing a Mtd treatment. Results on total Mtd determination showed a very poor correlation between saliva and serum, whereas the enantiomeric ratios of Mtd gave a very good one.
Keywords Chromatography, Liquid/methodsHumansMass SpectrometryMethadone/analysis/urineReproducibility of ResultsSaliva/chemistrySensitivity and SpecificitySpectrophotometry, Ultraviolet
PMID: 10735296
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ORTELLI, Didier et al. Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry. In: Journal of chromatography, 2000, vol. 871, n° 1-2, p. 163-72. doi: 10.1016/S0021-9673(99)01001-8 https://archive-ouverte.unige.ch/unige:5071

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Deposited on : 2010-01-28

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