Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry

Ortelli, Didier; Rudaz, Serge; Chevalley, Anne-Françoise; Mino, Annie; Deglon, Jean-Jacques; Balant, Luc; Veuthey, Jean-Luc

doi:10.1016/S0021-9673(99)01001-8

Scientific article

English

Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry

Contributors

Ortelli, Didier; Rudaz, Serge

; Chevalley, Anne-Françoise; Mino, Annie; Deglon, Jean-Jacques; Balant, Luc; Veuthey, Jean-Luc

Published in

Journal of chromatography, vol. 871, no. 1-2, p. 163-172

Publication date

2000

Abstract

Saliva was tested and evaluated as a biological matrix for methadone (Mtd) monitoring. Conventional method using a narrow bore C18 column, and an enantioselective method using a narrow bore alpha1-acid glycoprotein column, were developed using liquid chromatography coupled with a mass spectromeric (MS) detector. After optimisation of MS conditions by flow injection analysis, selected ion monitoring detection was used to enhance sensitivity. The total Mtd concentration and the enantiomeric ratio in saliva were validated using an experimental design. The methods were applied to samples provided by heroin addicts undergoing a Mtd treatment. Results on total Mtd determination showed a very poor correlation between saliva and serum, whereas the enantiomeric ratios of Mtd gave a very good one.

Keywords

Chromatography, Liquid/methods
Humans
Mass Spectrometry
Methadone/analysis/urine
Reproducibility of Results
Saliva/chemistry
Sensitivity and Specificity
Spectrophotometry, Ultraviolet

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

ORTELLI, Didier et al. Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry. In: Journal of chromatography, 2000, vol. 871, n° 1-2, p. 163–172. doi: 10.1016/S0021-9673(99)01001-8

Article (Published version)

Identifiers

PID : unige:5071
DOI : 10.1016/S0021-9673(99)01001-8
PMID : 10735296

Journal ISSN

0021-9673

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Creation

28/01/2010 10:37:00

First validation

28/01/2010 10:37:00

Update

14/03/2023 15:23:30

Status update

14/03/2023 15:23:30

Last indexation

18/03/2025 09:17:52

Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation