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Lipophilicity determination of highly lipophilic compounds by liquid chromatography

Published in Chemistry & biodiversity. 2009, vol. 6, no. 11, p. 1828-36
Abstract Different experimental strategies using short columns in both conventional liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) were evaluated to allow, for the first time with these techniques, the lipophilicity determination of compounds with log P>5. Various organic modifiers, stationary phases, and elution modes were tested on 14 rigid compounds with a CLogP between 5 and 8, and 38 compounds with log P(oct) from 0 to 5. The best results in HPLC were obtained with the 20-mm Discovery RP Amide C16 stationary phase in isocratic mode using MeOH as organic modifier. To improve analysis time, the UHPLC approach was then evaluated. Consequently, a generic method was developed with a 30-mm Acquity BEH Shield RP18 column in gradient mode using MeOH as organic modifier, allowing a fourfold gain of time compared to the HPLC method, for the highly lipophilic compounds tested. Finally, the most rapid and accurate results were obtained with a 10-mm Hypersil GOLD Javelin HTS stationary phase in UHPLC, enabling an eightfold gain of time compared to the HPLC method.
Keywords Lipophilic determinationLiquid chromatographyLipophilicity
PMID: 19937824
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GUILLOT, Amandine et al. Lipophilicity determination of highly lipophilic compounds by liquid chromatography. In: Chemistry & biodiversity, 2009, vol. 6, n° 11, p. 1828-36. doi: 10.1002/cbdv.200900115 https://archive-ouverte.unige.ch/unige:4718

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Deposited on : 2010-01-11

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