UNIGE document Scientific Article
previous document  unige:4718  next document
add to browser collection
Title

Lipophilicity determination of highly lipophilic compounds by liquid chromatography

Authors
Published in Chemistry and Biodiversity. 2009, vol. 6, no. 11, p. 1828-36
Abstract Different experimental strategies using short columns in both conventional liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) were evaluated to allow, for the first time with these techniques, the lipophilicity determination of compounds with log P>5. Various organic modifiers, stationary phases, and elution modes were tested on 14 rigid compounds with a CLogP between 5 and 8, and 38 compounds with log P(oct) from 0 to 5. The best results in HPLC were obtained with the 20-mm Discovery RP Amide C16 stationary phase in isocratic mode using MeOH as organic modifier. To improve analysis time, the UHPLC approach was then evaluated. Consequently, a generic method was developed with a 30-mm Acquity BEH Shield RP18 column in gradient mode using MeOH as organic modifier, allowing a fourfold gain of time compared to the HPLC method, for the highly lipophilic compounds tested. Finally, the most rapid and accurate results were obtained with a 10-mm Hypersil GOLD Javelin HTS stationary phase in UHPLC, enabling an eightfold gain of time compared to the HPLC method.
Keywords Lipophilic determinationLiquid chromatographyLipophilicity
Identifiers
PMID: 19937824
Full text
Structures
Citation
(ISO format)
GUILLOT, Amandine et al. Lipophilicity determination of highly lipophilic compounds by liquid chromatography. In: Chemistry and Biodiversity, 2009, vol. 6, n° 11, p. 1828-36. https://archive-ouverte.unige.ch/unige:4718

126 hits

2 downloads

Update

Deposited on : 2010-01-11

Export document
Format :
Citation style :