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Title

Simultaneous LC-MS/MS quantification of P-glycoprotein and cytochrome P450 probe substrates and their metabolites in DBS and plasma

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Published in Bioanalysis. 2014, vol. 6, no. 2, p. 151-64
Abstract An LC-MS/MS method has been developed for the simultaneous quantification of P-glycoprotein (P-gp) and cytochrome P450 (CYP) probe substrates and their Phase I metabolites in DBS and plasma. P-gp (fexofenadine) and CYP-specific substrates (caffeine for CYP1A2, bupropion for CYP2B6, flurbiprofen for CYP2C9, omeprazole for CYP2C19, dextromethorphan for CYP2D6 and midazolam for CYP3A4) and their metabolites were extracted from DBS (10 µl) using methanol. Analytes were separated on a reversed-phase LC column followed by SRM detection within a 6 min run time.
Keywords AnimalsBupropion/blood/metabolism/pharmacokineticsCaffeine/blood/metabolism/pharmacokineticsCalibrationChromatography, High Pressure Liquid/standardsCytochrome P-450 Enzyme System/metabolismDextromethorphan/blood/metabolism/pharmacokineticsDried Blood Spot TestingFlurbiprofen/blood/metabolism/pharmacokineticsHalf-LifeMaleMidazolam/blood/metabolism/pharmacokineticsOmeprazole/blood/metabolism/pharmacokineticsP-Glycoprotein/metabolismPharmaceutical Preparations/blood/metabolismSubstrate SpecificityTandem Mass Spectrometry/standardsTerfenadine/analogs & derivatives/blood/metabolism/pharmacokinetics
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PMID: 24423593
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Research groups Groupe Desmeules Jules (pharmacologie/toxicologie) (567)
Toxicologie analytique et forensique (120)
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(ISO format)
BOSILKOVSKA WEISSKOPF, Marija et al. Simultaneous LC-MS/MS quantification of P-glycoprotein and cytochrome P450 probe substrates and their metabolites in DBS and plasma. In: Bioanalysis, 2014, vol. 6, n° 2, p. 151-64. https://archive-ouverte.unige.ch/unige:43679

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Deposited on : 2014-12-17

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