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Title

Simultaneous LC-MS/MS quantification of P-glycoprotein and cytochrome P450 probe substrates and their metabolites in DBS and plasma
Authors
Bosilkovska Weisskopf, Marija
Deglon, Julien
Samer, Caroline Flora
Walder, Bernhard
Desmeules, Jules Alexandre
Staub, Christian
Daali, Youssef
Published in Bioanalysis. 2014, vol. 6, no. 2, p. 151-64
Abstract An LC-MS/MS method has been developed for the simultaneous quantification of P-glycoprotein (P-gp) and cytochrome P450 (CYP) probe substrates and their Phase I metabolites in DBS and plasma. P-gp (fexofenadine) and CYP-specific substrates (caffeine for CYP1A2, bupropion for CYP2B6, flurbiprofen for CYP2C9, omeprazole for CYP2C19, dextromethorphan for CYP2D6 and midazolam for CYP3A4) and their metabolites were extracted from DBS (10 µl) using methanol. Analytes were separated on a reversed-phase LC column followed by SRM detection within a 6 min run time.
Keywords Animals — Bupropion/blood/metabolism/pharmacokinetics — Caffeine/blood/metabolism/pharmacokinetics — Calibration — Chromatography, High Pressure Liquid/standards — Cytochrome P-450 Enzyme System/metabolism — Dextromethorphan/blood/metabolism/pharmacokinetics — Dried Blood Spot Testing — Flurbiprofen/blood/metabolism/pharmacokinetics — Half-Life — Male — Midazolam/blood/metabolism/pharmacokinetics — Omeprazole/blood/metabolism/pharmacokinetics — P-Glycoprotein/metabolism — Pharmaceutical Preparations/blood/metabolism — Substrate Specificity — Tandem Mass Spectrometry/standards — Terfenadine/analogs & derivatives/blood/metabolism/pharmacokinetics
Identifiers
PMID: 24423593
Full text
Article (Published version) (1.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie et soins intensifs
Faculté de médecine / Section de médecine clinique / Département de santé et de médecine communautaires / Institut de médecine légale
Research groups Groupe Desmeules Jules (pharmacologie/toxicologie) (567)
Toxicologie analytique et forensique (120)
Citation
(ISO format) 		BOSILKOVSKA WEISSKOPF, Marija et al. Simultaneous LC-MS/MS quantification of P-glycoprotein and cytochrome P450 probe substrates and their metabolites in DBS and plasma. In: Bioanalysis, 2014, vol. 6, n° 2, p. 151-64. https://archive-ouverte.unige.ch/unige:43679

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Deposited on : 2014-12-17

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