en
Scientific article
Open access
English

Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite

Published inJournal of pharmaceutical and biomedical analysis, vol. 54, no. 2, p. 359-367
Publication date2011
Abstract

This paper illustrates the development of an automated system for the on-line bioanalysis of dried blood spots (on-line DBS). In this way, a prototype was designed for integration into a conventional LC/MS/MS, allowing the successive extraction of 30 DBS toward the analytical system without any sample pretreatment. The developed method was assessed for the DBS analysis of flurbiprofen (FLB) and its metabolite 4-hydroxyflurbiprofen (OH-FLB) in human whole blood (i.e. 5 μL). The automated procedure was fully validated based on international criteria and showed good precision, trueness, and linearity over the expected concentration range (from 10 to 1000 ng/mL and 100 to 10,000 ng/mL for OH-FLB and FLB respectively). Furthermore, the prototype showed good results in terms of recovery and carry-over. Stability of both analytes on filter paper was also investigated and the results suggested that DBS could be stored at ambient temperature for over 1 month. The on-line DBS automated system was then successfully applied to a pharmacokinetic study performed on healthy male volunteers after oral administration of a single 50-mg dose of FLB. Additionally, a comparison between finger capillary DBS and classic venous plasma concentrations was investigated. A good correlation was observed, demonstrating the complementarity of both sampling forms. The automated system described in this article represents an efficient tool for the LC/MS/MS analysis of DBS samples in many bioanalytical applications.

Keywords
  • Administration, Oral
  • Adsorption
  • Blood Chemical Analysis/methods
  • Calibration
  • Chromatography, Liquid/methods
  • Desiccation
  • Drug Stability
  • Drug Storage
  • Flurbiprofen/administration & dosage/blood/pharmacokinetics
  • Guidelines as Topic
  • Humans
  • Male
  • Micropore Filters
  • Online Systems
  • Quality Control
  • Reference Standards
  • Reproducibility of Results
  • Specimen Handling/methods
  • Tablets
  • Tandem Mass Spectrometry/methods
Citation (ISO format)
DEGLON, Julien et al. Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite. In: Journal of pharmaceutical and biomedical analysis, 2011, vol. 54, n° 2, p. 359–367. doi: 10.1016/j.jpba.2010.08.032
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Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0731-7085
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650downloads

Technical informations

Creation11/19/2014 10:05:00 AM
First validation11/19/2014 10:05:00 AM
Update time03/14/2023 10:16:39 PM
Status update03/14/2023 10:16:39 PM
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