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Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite

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Published in Journal of pharmaceutical and biomedical analysis. 2011, vol. 54, no. 2, p. 359-67
Abstract This paper illustrates the development of an automated system for the on-line bioanalysis of dried blood spots (on-line DBS). In this way, a prototype was designed for integration into a conventional LC/MS/MS, allowing the successive extraction of 30 DBS toward the analytical system without any sample pretreatment. The developed method was assessed for the DBS analysis of flurbiprofen (FLB) and its metabolite 4-hydroxyflurbiprofen (OH-FLB) in human whole blood (i.e. 5 μL). The automated procedure was fully validated based on international criteria and showed good precision, trueness, and linearity over the expected concentration range (from 10 to 1000 ng/mL and 100 to 10,000 ng/mL for OH-FLB and FLB respectively). Furthermore, the prototype showed good results in terms of recovery and carry-over. Stability of both analytes on filter paper was also investigated and the results suggested that DBS could be stored at ambient temperature for over 1 month. The on-line DBS automated system was then successfully applied to a pharmacokinetic study performed on healthy male volunteers after oral administration of a single 50-mg dose of FLB. Additionally, a comparison between finger capillary DBS and classic venous plasma concentrations was investigated. A good correlation was observed, demonstrating the complementarity of both sampling forms. The automated system described in this article represents an efficient tool for the LC/MS/MS analysis of DBS samples in many bioanalytical applications.
Keywords Administration, OralAdsorptionBlood Chemical Analysis/methodsCalibrationChromatography, Liquid/methodsDesiccationDrug StabilityDrug StorageFlurbiprofen/administration & dosage/blood/pharmacokineticsGuidelines as TopicHumansMaleMicropore FiltersOnline SystemsQuality ControlReference StandardsReproducibility of ResultsSpecimen Handling/methodsTabletsTandem Mass Spectrometry/methods
PMID: 20864289
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Article (Published version) (678 Kb) - public document Free access
Research groups Groupe Desmeules Jules (pharmacologie/toxicologie) (567)
Pharmaco-omiques et médecine de précision (1003)
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DEGLON, Julien et al. Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite. In: Journal of pharmaceutical and biomedical analysis, 2011, vol. 54, n° 2, p. 359-67. doi: 10.1016/j.jpba.2010.08.032 https://archive-ouverte.unige.ch/unige:42027

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Deposited on : 2014-11-20

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