UNIGE document Scientific Article
previous document  unige:32858  next document
add to browser collection
Title

Comparison of gel-based methods for the detection of cerebrospinal fluid rhinorrhea

Authors
Published in Clinica Chimica Acta. 2012, vol. 413, no. 13-14, p. 1145-50
Abstract Cerebrospinal fluid (CSF) rhinorrhea is a serious condition that may result in severe complications. Various laboratory tests, relying on the detection of CSF-specific proteins in nasal secretions, have been developed but diagnosis remains challenging. The aim of this study was to evaluate two new methods targeting either ß2-transferrin or beta-trace-protein. Rhinorrhea samples from patients suspected of CSF leakage (n=36) were analyzed using two-dimensional gel electrophoresis (2-DE) for CSF rhinorrhea diagnosis. Twelve patients with rhinorrhea strongly suggestive of a CSF leak also underwent a fluorescein test. The same cohort was retrospectively analyzed with a beta-trace protein immunoblot developed in-house (n=36) and a new commercial ß2-transferrin immunofixation assay (Sebia, Evry, France) (n=33). 2-DE was positive in 9 patients suffering from rhinorrhea following skull base fracture (n=3), post-surgery (n=4), or spontaneously (n=2). The 27 remaining cases were negative. These results were confirmed by the beta-trace protein immunoblot and ß2-transferrin immunofixation tests, except for one sample found negative with 2-DE but positive with the two other assays. Results from the three analytical methods were concordant with fluorescein tests. Beta-trace protein immunoblot and ß2-transferrin immunofixation assays are fast and reliable methods that allow detecting CSF leakage in nasal fluid with high sensitivity and specificity.
Keywords AdolescentAdultAgedAged, 80 and overCerebrospinal Fluid Rhinorrhea/diagnosisFemaleHumansImmunoblotting/methodsImmunoelectrophoresis/methodsIntramolecular Oxidoreductases/analysisLipocalins/analysisMaleMiddle AgedTransferrin/analysisYoung Adult
Identifiers
PMID: 22483794
Full text
Article (Published version) (365 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Groupe Pierre Burkhard (neurologie) (154)
Chimie et protéomique clinique (270)
Citation
(ISO format)
LESCUYER, Pierre et al. Comparison of gel-based methods for the detection of cerebrospinal fluid rhinorrhea. In: Clinica Chimica Acta, 2012, vol. 413, n° 13-14, p. 1145-50. https://archive-ouverte.unige.ch/unige:32858

137 hits

0 download

Update

Deposited on : 2014-01-07

Export document
Format :
Citation style :