Comparison of gel-based methods for the detection of cerebrospinal fluid rhinorrhea

Article Article scientifique Article Comparison of gel-based methods for the detection of cerebrospinal fluid rhinorrhea eng 2012 author Lescuyer Pierre 0000-0001-6986-8738 562350 author Auer Lucas 47963 author Converset Véronique 628081 author Hochstrasser Denis 26422 author Landis Basile Nicolas 133537 author Burkhard Pierre 0000-0002-7459-5778 92389 1145-1150 10.1016/j.cca.2012.03.016 22483794 0009-8981 Cerebrospinal fluid (CSF) rhinorrhea is a serious condition that may result in severe complications. Various laboratory tests, relying on the detection of CSF-specific proteins in nasal secretions, have been developed but diagnosis remains challenging. The aim of this study was to evaluate two new methods targeting either ß2-transferrin or beta-trace-protein. Rhinorrhea samples from patients suspected of CSF leakage (n=36) were analyzed using two-dimensional gel electrophoresis (2-DE) for CSF rhinorrhea diagnosis. Twelve patients with rhinorrhea strongly suggestive of a CSF leak also underwent a fluorescein test. The same cohort was retrospectively analyzed with a beta-trace protein immunoblot developed in-house (n=36) and a new commercial ß2-transferrin immunofixation assay (Sebia, Evry, France) (n=33). 2-DE was positive in 9 patients suffering from rhinorrhea following skull base fracture (n=3), post-surgery (n=4), or spontaneously (n=2). The 27 remaining cases were negative. These results were confirmed by the beta-trace protein immunoblot and ß2-transferrin immunofixation tests, except for one sample found negative with 2-DE but positive with the two other assays. Results from the three analytical methods were concordant with fluorescein tests. Beta-trace protein immunoblot and ß2-transferrin immunofixation assays are fast and reliable methods that allow detecting CSF leakage in nasal fluid with high sensitivity and specificity. Adolescent Adult Aged Aged, 80 and over Cerebrospinal Fluid Rhinorrhea/diagnosis Female Humans Immunoblotting/methods Immunoelectrophoresis/methods Intramolecular Oxidoreductases/analysis Lipocalins/analysis Male Middle Aged Transferrin/analysis Young Adult Dewey 576 Dewey 616.8 136 Département de biologie structurale et bioinformatique 21E 61 Département des neurosciences cliniques 226 false 211 Auto-immunité, inflammation et athérosclérose 270 124 Groupe Pierre Burkhard (neurologie) 154 Clinica chimica acta 413 13-14 52334192-041b-46f0-a867-fd3e112c9a77 Article (Published version) Restricted 1-s2.0-S000989811200143X-main.pdf 373882 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

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Comparison of gel-based methods for the detection of cerebrospinal fluid rhinorrhea	Clinica chimica acta			2012	610	0

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Auer, Lucas