Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth

Scherl, Alexander; Francois, Patrice; Bento, Manuela; Deshusses, Jacques; Charbonnier, Yvan; Converset, Véronique; Huyghe, Antoine; Walter, Nadia; Hoogland, Christine; Appel, Ron David; Sanchez, Jean-Charles; Zimmermann, Catherine; Corthals, Garry; Hochstrasser, Denis; Schrenzel, Jacques

doi:10.1016/j.mimet.2004.09.017

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Title		Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth
Authors		Scherl, Alexander Francois, Patrice Bento, Manuela Deshusses, Jacques Charbonnier, Yvan Converset, Véronique Huyghe, Antoine Walter, Nadia Hoogland, Christine Appel, Ron David Sanchez, Jean-Charles Zimmermann, Catherine Corthals, Garry Hochstrasser, Denis Schrenzel, Jacques show all authors [1 - 15]
Published in		Journal of Microbiological Methods. 2005, vol. 60, no. 2, p. 247-257
Abstract		A combined proteomic and transcriptomic analysis of Staphylococcus aureus strain N315 was performed to study a sequenced strain at the system level. Total protein and membrane protein extracts were prepared and analyzed using various proteomic workflows including: 2-DE, SDS-PAGE combined with microcapillary LC-MALDI-MS/MS, and multidimensional liquid chromatography. The presence of a protein was then correlated with its respective transcript level from S. aureus cells grown under the same conditions. Gene-expression data revealed that 97% of the 2'596 ORFs were detected during the post-exponential phase. At the protein level, 23% of these ORFs (591 proteins) were identified. Correlation of the two datasets revealed that 42% of the identified proteins (248 proteins) were amongst the top 25% of genes with highest mRNA signal intensities, and 69% of the identified proteins (406 proteins) were amongst the top 50% with the highest mRNA signal intensities. The fact that the remaining 31% of proteins were not strongly expressed at the RNA level indicates either that some low-abundance proteins were identified or that some transcripts or proteins showed extended half-lives. The most abundant classes identified with the combined proteomic and transcriptomic approach involved energy production, translational activities and nucleotide transport, reflecting an active metabolism. The simultaneous large-scale analysis of transcriptomes and proteomes enables a global and holistic view of the S. aureus biology, allowing the parallel study of multiple active events in an organism.
Keywords		Bacterial Proteins/biosynthesis/genetics/isolation & purification — Chromatography, Liquid — Computational Biology — Electrophoresis, Gel, Two-Dimensional — Electrophoresis, Polyacrylamide Gel — Gene Expression Regulation, Developmental — Nucleic Acid Hybridization — Oligonucleotide Array Sequence Analysis — Proteomics/methods — RNA, Messenger/genetics/isolation & purification — Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization — Staphylococcus aureus/genetics/growth & development/metabolism — Transcription, Genetic/genetics/physiology
Identifiers		DOI: 10.1016/j.mimet.2004.09.017 PMID: 15590099
Full text		Article (Published version) (354 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine Faculté des sciences / Département d'informatique Centres et instituts / Centre universitaire d'informatique Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research group		Groupe de Protéomique biomédicale (635)
Citation (ISO format)		SCHERL, Alexander et al. Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth. In: Journal of Microbiological Methods, 2005, vol. 60, n° 2, p. 247-257. https://archive-ouverte.unige.ch/unige:32159