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Title

Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth

Authors
Bento, Manuela
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Published in Journal of Microbiological Methods. 2005, vol. 60, no. 2, p. 247-257
Abstract A combined proteomic and transcriptomic analysis of Staphylococcus aureus strain N315 was performed to study a sequenced strain at the system level. Total protein and membrane protein extracts were prepared and analyzed using various proteomic workflows including: 2-DE, SDS-PAGE combined with microcapillary LC-MALDI-MS/MS, and multidimensional liquid chromatography. The presence of a protein was then correlated with its respective transcript level from S. aureus cells grown under the same conditions. Gene-expression data revealed that 97% of the 2'596 ORFs were detected during the post-exponential phase. At the protein level, 23% of these ORFs (591 proteins) were identified. Correlation of the two datasets revealed that 42% of the identified proteins (248 proteins) were amongst the top 25% of genes with highest mRNA signal intensities, and 69% of the identified proteins (406 proteins) were amongst the top 50% with the highest mRNA signal intensities. The fact that the remaining 31% of proteins were not strongly expressed at the RNA level indicates either that some low-abundance proteins were identified or that some transcripts or proteins showed extended half-lives. The most abundant classes identified with the combined proteomic and transcriptomic approach involved energy production, translational activities and nucleotide transport, reflecting an active metabolism. The simultaneous large-scale analysis of transcriptomes and proteomes enables a global and holistic view of the S. aureus biology, allowing the parallel study of multiple active events in an organism.
Keywords Bacterial Proteins/biosynthesis/genetics/isolation & purificationChromatography, LiquidComputational BiologyElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelGene Expression Regulation, DevelopmentalNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisProteomics/methodsRNA, Messenger/genetics/isolation & purificationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStaphylococcus aureus/genetics/growth & development/metabolismTranscription, Genetic/genetics/physiology
Identifiers
PMID: 15590099
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Research group Groupe de Protéomique biomédicale (635)
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SCHERL, Alexander et al. Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth. In: Journal of Microbiological Methods, 2005, vol. 60, n° 2, p. 247-257. https://archive-ouverte.unige.ch/unige:32159

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Deposited on : 2013-12-16

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