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Scientific article
English

Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth

Contributors

Scherl, Alexander; Francois, Patrice; Bento, Manuela; Deshusses, Jacques; Charbonnier, Yvan; Converset, Véronique; Huyghe, Antoine; Walter, Nadia; Hoogland, Christine; Appel, Ron David; Sanchez, Jean-Charles; Zimmermann, Catherine; Corthals, Garry; Hochstrasser, Denis; Schrenzel, Jacques

Published in

Journal of microbiological methods, vol. 60, no. 2, p. 247-257

Publication date

2005

Abstract

A combined proteomic and transcriptomic analysis of Staphylococcus aureus strain N315 was performed to study a sequenced strain at the system level. Total protein and membrane protein extracts were prepared and analyzed using various proteomic workflows including: 2-DE, SDS-PAGE combined with microcapillary LC-MALDI-MS/MS, and multidimensional liquid chromatography. The presence of a protein was then correlated with its respective transcript level from S. aureus cells grown under the same conditions. Gene-expression data revealed that 97% of the 2'596 ORFs were detected during the post-exponential phase. At the protein level, 23% of these ORFs (591 proteins) were identified. Correlation of the two datasets revealed that 42% of the identified proteins (248 proteins) were amongst the top 25% of genes with highest mRNA signal intensities, and 69% of the identified proteins (406 proteins) were amongst the top 50% with the highest mRNA signal intensities. The fact that the remaining 31% of proteins were not strongly expressed at the RNA level indicates either that some low-abundance proteins were identified or that some transcripts or proteins showed extended half-lives. The most abundant classes identified with the combined proteomic and transcriptomic approach involved energy production, translational activities and nucleotide transport, reflecting an active metabolism. The simultaneous large-scale analysis of transcriptomes and proteomes enables a global and holistic view of the S. aureus biology, allowing the parallel study of multiple active events in an organism.

Keywords

  • Bacterial Proteins/biosynthesis/genetics/isolation & purification
  • Chromatography, Liquid
  • Computational Biology
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Developmental
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Proteomics/methods
  • RNA, Messenger/genetics/isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Staphylococcus aureus/genetics/growth & development/metabolism
  • Transcription, Genetic/genetics/physiology

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

SCHERL, Alexander et al. Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth. In: Journal of microbiological methods, 2005, vol. 60, n° 2, p. 247–257. doi: 10.1016/j.mimet.2004.09.017

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0167-7012
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Technical informations

Creation

15/11/2013 13:15:00

First validation

15/11/2013 13:15:00

Update

09/01/2025 17:05:50

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