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New pool of cortical interneuron precursors in the early postnatal dorsal white matter

Murthy, Sahana
Szabo, G
Vitalis, T
Published in Cerebral cortex. 2012, vol. 22, no. 1, p. 86-98
Abstract The migration of cortical γ-aminobutyric acidergic interneurons has been extensively studied in rodent embryos, whereas few studies have documented their postnatal migration. Combining in vivo analysis together with time-lapse imaging on cortical slices, we explored the origin and migration of cortical interneurons during the first weeks of postnatal life. Strikingly, we observed that a large pool of GAD65-GFP-positive cells accumulate in the dorsal white matter region during the first postnatal week. Part of these cells divides and expresses the transcription factor paired box 6 indicating the presence of local transient amplifying precursors. The vast majority of these cells are immature interneurons expressing the neuronal marker doublecortin and partly the calcium-binding protein calretinin. Time-lapse imaging reveals that GAD65-GFP-positive neurons migrate from the white matter pool into the overlying anterior cingulate cortex (aCC). Some interneurons in the postnatal aCC express the same immature neuronal markers suggesting ongoing migration of calretinin-positive interneurons. Finally, bromodeoxyuridine incorporation experiments confirm that a small fraction of interneurons located in the aCC are generated during the early postnatal period. These results altogether reveal that at postnatal ages, the dorsal white matter contains a pool of interneuron precursors that divide and migrate into the aCC.
Keywords Age FactorsAnimalsAnimals, NewbornBromodeoxyuridine/metabolismCell Movement/geneticsCell ProliferationCerebral Cortex/cytology/growth & developmentEmbryo, MammalianEye ProteinsFemaleGABAergic Neurons/physiologyGene Expression Regulation, Developmental/genetics/physiologyGlutamate Decarboxylase/genetics/metabolismHomeodomain ProteinsInterneurons/physiologyKi-67 Antigen/genetics/metabolismLuminescent Proteins/genetics/metabolismMiceMice, Inbred C57BLMice, TransgenicNerve Fibers, Myelinated/metabolism/physiologyNerve Tissue Proteins/genetics/metabolismNuclear Proteins/genetics/metabolismPaired Box Transcription FactorsPregnancyProteins/geneticsReceptors, Serotonin, 5-HT3/geneticsRepressor ProteinsTranscription Factors/genetics/metabolism
PMID: 21616983
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Research groups Anesthésie et neurosciences (883)
Groupe Dayer Alexandre (Formation du circuit cortical) (875)
Progéniteurs neuronaux
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RICCIO WICHT, Orbicia et al. New pool of cortical interneuron precursors in the early postnatal dorsal white matter. In: Cerebral cortex, 2012, vol. 22, n° 1, p. 86-98. doi: 10.1093/cercor/bhr086 https://archive-ouverte.unige.ch/unige:31849

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Deposited on : 2013-12-06

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