en
Scientific article
English

New pool of cortical interneuron precursors in the early postnatal dorsal white matter

Published inCerebral cortex, vol. 22, no. 1, p. 86-98
Publication date2012
Abstract

The migration of cortical γ-aminobutyric acidergic interneurons has been extensively studied in rodent embryos, whereas few studies have documented their postnatal migration. Combining in vivo analysis together with time-lapse imaging on cortical slices, we explored the origin and migration of cortical interneurons during the first weeks of postnatal life. Strikingly, we observed that a large pool of GAD65-GFP-positive cells accumulate in the dorsal white matter region during the first postnatal week. Part of these cells divides and expresses the transcription factor paired box 6 indicating the presence of local transient amplifying precursors. The vast majority of these cells are immature interneurons expressing the neuronal marker doublecortin and partly the calcium-binding protein calretinin. Time-lapse imaging reveals that GAD65-GFP-positive neurons migrate from the white matter pool into the overlying anterior cingulate cortex (aCC). Some interneurons in the postnatal aCC express the same immature neuronal markers suggesting ongoing migration of calretinin-positive interneurons. Finally, bromodeoxyuridine incorporation experiments confirm that a small fraction of interneurons located in the aCC are generated during the early postnatal period. These results altogether reveal that at postnatal ages, the dorsal white matter contains a pool of interneuron precursors that divide and migrate into the aCC.

Keywords
  • Age Factors
  • Animals
  • Animals, Newborn
  • Bromodeoxyuridine/metabolism
  • Cell Movement/genetics
  • Cell Proliferation
  • Cerebral Cortex/cytology/growth & development
  • Embryo, Mammalian
  • Eye Proteins
  • Female
  • GABAergic Neurons/physiology
  • Gene Expression Regulation, Developmental/genetics/physiology
  • Glutamate Decarboxylase/genetics/metabolism
  • Homeodomain Proteins
  • Interneurons/physiology
  • Ki-67 Antigen/genetics/metabolism
  • Luminescent Proteins/genetics/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Nerve Fibers, Myelinated/metabolism/physiology
  • Nerve Tissue Proteins/genetics/metabolism
  • Nuclear Proteins/genetics/metabolism
  • Paired Box Transcription Factors
  • Pregnancy
  • Proteins/genetics
  • Receptors, Serotonin, 5-HT3/genetics
  • Repressor Proteins
  • Transcription Factors/genetics/metabolism
Citation (ISO format)
RICCIO WICHT, Orbicia et al. New pool of cortical interneuron precursors in the early postnatal dorsal white matter. In: Cerebral cortex, 2012, vol. 22, n° 1, p. 86–98. doi: 10.1093/cercor/bhr086
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1047-3211
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