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Title

Human hemolysate glycated proteome

Authors
Priego-Capote, Feliciano
Ramirez-Boo, Maria
Published in Analytical Chemistry. 2011, vol. 83, no. 14, p. 5673-80
Abstract Despite continuous advances in hyperglycemia treatments, a precise control through monitoring of glucose and glycated hemoglobin remains in most diabetic patients as the diagnosis/prognosis tool. An alternative perspective could be the discovery and quantitation of new blood glycated proteins formed by nonenzymatic reaction with circulatory glucose. As a result, the human hemolysate is an incomparable source of glycated proteins to further monitor glycemia and interpret changes at the level of this post-translational modification. The human hemolysate is here studied based on the differential labeling of proteins with isotopically labeled-glucose ([(13)C(6)] glucose), named glycation isotopic labeling. Due to the chemoselectivity of glycation, only preferential targets are labeled by this protocol. The approach provides qualitative data through the detection of preferential protein glycation sites as well as quantitative information to evaluate the abundance of this modification. This strategy was applied to human hemolysate samples corresponding to different glycemic states estimated by laboratory-certified concentrations of glycated hemoglobin. The glycation level of each protein can then be employed to interpret the effect of glucose exposition as a consequence of glycemic unbalance. This information should provide new molecular insights into protein glycation mechanisms that might generate a new hypothesis to clinicians to improve the understanding of underlying pathologies associated to prolonged hyperglycemia.
Keywords Glucose/metabolismGlycosylationHemoglobin A, Glycosylated/metabolismHemolysisHumansProteome/metabolism
Identifiers
PMID: 21630644
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Research group Groupe de Protéomique biomédicale (635)
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PRIEGO-CAPOTE, Feliciano et al. Human hemolysate glycated proteome. In: Analytical Chemistry, 2011, vol. 83, n° 14, p. 5673-80. https://archive-ouverte.unige.ch/unige:25573

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Deposited on : 2013-01-16

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