Human hemolysate glycated proteome

Article Article scientifique Article Human hemolysate glycated proteome eng 2011 author Priego-Capote Feliciano author Ramirez-Boo Maria author Hoogland Christine 503821 author Scherl Alexander 500181 author Mueller Markus 767011 author Lisacek Frédérique 0000-0002-0948-4537 639315 author Sanchez Jean-Charles 0000-0002-8733-5932 24399 5673-5680 10.1021/ac200864b 21630644 0003-2700 Despite continuous advances in hyperglycemia treatments, a precise control through monitoring of glucose and glycated hemoglobin remains in most diabetic patients as the diagnosis/prognosis tool. An alternative perspective could be the discovery and quantitation of new blood glycated proteins formed by nonenzymatic reaction with circulatory glucose. As a result, the human hemolysate is an incomparable source of glycated proteins to further monitor glycemia and interpret changes at the level of this post-translational modification. The human hemolysate is here studied based on the differential labeling of proteins with isotopically labeled-glucose ([(13)C(6)] glucose), named glycation isotopic labeling. Due to the chemoselectivity of glycation, only preferential targets are labeled by this protocol. The approach provides qualitative data through the detection of preferential protein glycation sites as well as quantitative information to evaluate the abundance of this modification. This strategy was applied to human hemolysate samples corresponding to different glycemic states estimated by laboratory-certified concentrations of glycated hemoglobin. The glycation level of each protein can then be employed to interpret the effect of glucose exposition as a consequence of glycemic unbalance. This information should provide new molecular insights into protein glycation mechanisms that might generate a new hypothesis to clinicians to improve the understanding of underlying pathologies associated to prolonged hyperglycemia. Glucose/metabolism Glycosylation Hemoglobin A, Glycosylated/metabolism Hemolysis Humans Proteome/metabolism Dewey 576 Dewey 615 31 Section des sciences pharmaceutiques 17 136 Département de biologie structurale et bioinformatique 21E false 369 Biomarqueurs 635 Analytical chemistry 83 14 670bf6c5-903a-42cf-9f31-88d1f199a01b Article (Published version) Restricted 21630644.pdf 880956 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique

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Human hemolysate glycated proteome	Analytical chemistry			2011	642	1

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Mueller, Markus