UNIGE document Scientific Article
previous document  unige:21624  next document
add to browser collection
Title

Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic–mass spectrometric

Authors
Published in Food Chemistry. 2012, vol. 132, no. 1, p. 613-618
Abstract The potential of solid-phase microextraction on polyacrylate coated fibre, with sequential or simultaneous trimethylsilyl derivatisation followed by gas chromatographic–mass spectrometric analysis, was evaluated for a rapid determination of the distribution of the phytosterols in aqueous food matrixes. Influences of different parameters (bis(trimethylsilyl)trifluoro-acetamide and sterol exposure time, sterol concentration and experimental protocol) on the recovery of sterols were investigated to determine optimum conditions which were tested for sterol extraction and analysis from orange juice. Best selectivity, sterol recovery and derivatisation yields were obtained by extraction and simultaneous derivatisation through immersion of the SPME-PA fibre in the orange juice (10 min, 65 °C) after headspace absorption of BSTFA (30 min, 65 °C) on the fibre. Nevertheless the method developed cannot be used for quantitative analysis. But the possibility to effect rapid screen of phytosterol containing in complex media have been shown.
Identifiers
Full text
Structures
Research group Groupe Gülaçar
Citation
(ISO format)
BALME, Sébastien Christian, GULACAR, Fazil. Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic–mass spectrometric. In: Food Chemistry, 2012, vol. 132, n° 1, p. 613-618. https://archive-ouverte.unige.ch/unige:21624

230 hits

1 download

Update

Deposited on : 2012-06-19

Export document
Format :
Citation style :