Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic

Article Article scientifique Article Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic–mass spectrometric eng 2012 author Balme Sébastien Christian 640619 author Gulacar Fazil 24667 613-618 10.1016/j.foodchem.2011.10.097 0308-8146 http://linkinghub.elsevier.com/retrieve/pii/S0308814611015688 The potential of solid-phase microextraction on polyacrylate coated fibre, with sequential or simultaneous trimethylsilyl derivatisation followed by gas chromatographic–mass spectrometric analysis, was evaluated for a rapid determination of the distribution of the phytosterols in aqueous food matrixes. Influences of different parameters (bis(trimethylsilyl)trifluoro-acetamide and sterol exposure time, sterol concentration and experimental protocol) on the recovery of sterols were investigated to determine optimum conditions which were tested for sterol extraction and analysis from orange juice. Best selectivity, sterol recovery and derivatisation yields were obtained by extraction and simultaneous derivatisation through immersion of the SPME-PA fibre in the orange juice (10 min, 65 °C) after headspace absorption of BSTFA (30 min, 65 °C) on the fibre. Nevertheless the method developed cannot be used for quantitative analysis. But the possibility to effect rapid screen of phytosterol containing in complex media have been shown. Dewey 540 38 Département de chimie physique 142 false 693 Groupe Gülaçar Food chemistry 132 1 148e8132-9f55-4b8d-bb90-5fe46920b00d Article (Published version) Restricted ref01298.pdf 690928 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie physique

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Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic–mass spectrometric	Food chemistry			2012	729	0

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Balme, Sébastien Christian