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Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children

Megevand, C.
Kind, C.
Gnehm, H-P
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Published in Clinical microbiology and infection. 2010, vol. 16, no. 9, p. 1414-1420
Abstract Nasal carriage of Staphylococcus aureus contributes to an increased risk of developing an infection with the same bacterial strain. Genetic regulatory elements and toxin-expressing genes are virulence factors associated with the pathogenic potential of S. aureus. We undertook an extensive molecular characterization of methicillin-susceptible S. aureus (MSSA) carried by children. MSSA were recovered from the nostrils of children. The presence of Panton-Valentine leukocidin (PVL), exfoliatins A and B (exfoA and exfoB), and the toxic-shock staphylococcal toxin (TSST-1) and agr group typing were determined by quantitative PCR. A multiple-locus variable-number of tandem repeat analysis (MLVA) assay was also performed for genotyping. Five hundred and seventy-two strains of MSSA were analysed. Overall, 30% were positive for toxin-expressing genes: 29% contained one toxin and 1.6% two toxins. The most commonly detected toxin gene was tst, which was present in 145 (25%) strains. The TSST-1 gene was significantly associated with the agr group 3 (OR 56.8, 95% CI 32.0-100.8). MLVA analysis revealed a large diversity of genetic content and no clonal relationship was demonstrated among the analysed MSSA strains. Multilocus sequence typing confirmed this observation of diversity and identified ST45 as a frequent colonizer. This broad diversity in MSSA carriage strains suggests a limited selection pressure in our geographical area.
Keywords Anti-Bacterial Agents/pharmacologyBacterial Toxins/geneticsBacterial Typing TechniquesCarrier State/*epidemiology/*microbiologyChildChild, PreschoolCluster AnalysisFemaleGenotypeHumansInfantMaleMethicillin/pharmacologyMinisatellite RepeatsMolecular EpidemiologyMolecular TypingNose/*microbiologyStaphylococcal Infections/*epidemiology/*microbiologyStaphylococcus aureus/*classification/genetics/*isolation & purificationSwitzerland/epidemiologyVirulence Factors/genetics
PMID: 19845693
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Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Etiologie des pneumonies et marqueurs inflammatoires chez l'enfant fébrile (183)
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MEGEVAND, C. et al. Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children. In: Clinical microbiology and infection, 2010, vol. 16, n° 9, p. 1414-1420. doi: 10.1111/j.1469-0691.2009.03090.x https://archive-ouverte.unige.ch/unige:21132

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Deposited on : 2012-05-23

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