Scientific article

Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedside

Published inCurrent opinion in infectious diseases, vol. 23, no. 4, p. 311-319
Publication date2010

PURPOSE OF REVIEW: Faster identification of infecting microorganisms and treatment options is a first-ranking priority in the infectious disease area, in order to prevent inappropriate treatment and overuse of broad-spectrum antibiotics. Standard bacterial identification is intrinsically time-consuming, and very recently there has been a burst in the number of commercially available nonphenotype-based techniques and in the documentation of a possible clinical impact of these techniques. RECENT FINDINGS: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now a standard diagnostic procedure on cultures and hold promises on spiked blood. Meanwhile, commercial PCR-based techniques have improved with the use of bacterial DNA enrichment methods, the diversity of amplicon analysis techniques (melting curve analysis, microarrays, gel electrophoresis, sequencing and analysis by mass spectrometry) leading to the ability to challenge bacterial culture as the gold standard for providing earlier diagnosis with a better 'clinical' sensitivity and additional prognostic information. SUMMARY: Laboratory practice has already changed with MALDI-TOF MS, but a change in clinical practice, driven by emergent nucleic acid-based techniques, will need the demonstration of real-life applicability as well as robust clinical-impact-oriented studies.

  • Bacteremia/*diagnosis/microbiology
  • *Bacterial Typing Techniques/methods/trends
  • DNA, Bacterial
  • Humans
  • Microbial Sensitivity Tests
  • *Molecular Diagnostic Techniques/methods/trends
  • Point-of-Care Systems
  • Polymerase Chain Reaction
  • Time Factors
Citation (ISO format)
LEGGIERI, N. et al. Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedside. In: Current opinion in infectious diseases, 2010, vol. 23, n° 4, p. 311–319. doi: 10.1097/QCO.0b013e32833bfc44
Main files (1)
ISSN of the journal0951-7375

Technical informations

Creation05/23/2012 8:49:58 AM
First validation05/23/2012 8:49:58 AM
Update time03/14/2023 5:34:06 PM
Status update03/14/2023 5:34:06 PM
Last indexation02/12/2024 8:15:02 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack