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Title

Concentration of GPI-anchored proteins upon ER exit in yeast

Authors
Watanabe, Reika
Published in Traffic. 2009, vol. 10, no. 2, p. 186-200
Abstract Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae, one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1, we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-alpha-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p.
Keywords Amino Acid Transport Systems/genetics/metabolismCell Wall/genetics/*metabolismCytoplasmic Vesicles/metabolismEndoplasmic Reticulum/*metabolismGlucose Transport Proteins, FacilitativeGlycosylphosphatidylinositols/*metabolismMembrane Glycoproteins/genetics/*metabolismMembrane Proteins/genetics/metabolismMonosaccharide Transport Proteins/genetics/metabolismSaccharomyces cerevisiae/genetics/*metabolismSaccharomyces cerevisiae Proteins/genetics/*metabolismTemperatureVesicular Transport Proteins/genetics/metabolism
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PMID: 19054390
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CASTILLON, Guillaume Alain et al. Concentration of GPI-anchored proteins upon ER exit in yeast. In: Traffic, 2009, vol. 10, n° 2, p. 186-200. https://archive-ouverte.unige.ch/unige:19729

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Deposited on : 2012-04-23

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